Lee, Hee Ju Lee, Eun Ha Jung, Sang Hoon Lee, Dong-Un Kim, Chul Young 2024-01-20T18:01:20Z 2024-01-20T18:01:20Z 2021-09-02 2011-01 1082-6076 https://pubs.kist.re.kr/handle/201004/130775 A pressurized liquid extraction (PLE) technique was developed for the extraction of bioactive isoflavonoids including tectoridin (1), iridin (2), tectorigenin (3), iristectorigenin A (4), irisflorentine (5), and irigenin (6) from Belamcanda chinensis using aqueous ethanol as the extraction solvent. The effects of various key factors of PLE, including solvent composition (0-100% ethanol in water), temperature (80-180 degrees C) and extraction time (5-20min), were evaluated with respect to the extraction efficiency. Only solvent compositions altered the extraction yields of isoflavonoids with 60% ethanol providing the best extraction efficiency. When compared with conventional extraction methods, such as reflux or sonication extraction, PLE resulted in higher extraction efficiency with shorter extraction time and lower solvent consumption. We also tested the thermal stability of tectoridin, isoflavonoid glycoside, at various temperatures (80-180 degrees C) in 60% ethanol for a 20min extraction time to mimic conditions that could be encountered during PLE. Tectoridin was degraded to tectorigenin (aglycone) at temperatures over 150 degrees C, therefore, care should be taken in the choice of temperature used for PLE. English TAYLOR & FRANCIS INC HOT-WATER EXTRACTION DIELECTRIC-CONSTANTS MEDICINAL-PLANTS CHROMATOGRAPHY TECTORIGENIN TEMPERATURE SOLVENT COMPOSITION EFFECTS ON EFFICIENCY OF PRESSURIZED LIQUID EXTRACTION OF BIOACTIVE ISOFLAVONOIDS FROM BELAMCANDA CHINENSIS RHIZOMES Article 10.1080/10826076.2011.540921 1 JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v.34, no.2, pp.143 - 154 JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 34 2 143 154 scie scopus 000286817200006 2-s2.0-78651353063 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article HOT-WATER EXTRACTION DIELECTRIC-CONSTANTS MEDICINAL-PLANTS CHROMATOGRAPHY TECTORIGENIN TEMPERATURE Belamcanda chinensis isoflavonoids pressurized liquid extraction tectoridin tectorigenin thermal stability