Min, Hye Kyeong
Lim, Sangsoo
Chung, Bong Chul
Moon, Myeong Hee
2024-01-20T18:01:48Z
2024-01-20T18:01:48Z
2021-09-02
2011-01
1618-2642
https://pubs.kist.re.kr/handle/201004/130799
Qualitative and quantitative profiling of six different categories of urinary phospholipids (PLs) from patients with prostate cancer was performed to develop an analytical method for the discovery of candidate biomarkers by shotgun lipidomics method. Using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry, we identified the molecular structures of a total of 70 PL molecules (21 phosphatidylcholines (PCs), 11 phosphatidylethanolamines (PEs), 17 phosphatidylserines (PSs), 11 phosphatidylinositols (PIs), seven phosphatidic acids, and three phosphatidylglycerols) from urine samples of healthy controls and prostate cancer patients by data-dependent collision-induced dissociation. Identified molecules were quantitatively examined by comparing the MS peak areas. From statistical analyses, one PC, one PE, six PSs, and two PIs among the PL species showed significant differences between controls and cancer patients (p < 0.05, Student's t test), with concentration changes of more than threefold. Cluster analysis of both control and patient groups showed that 18:0/18:1-PS and 16:0/22:6-PS were 99% similar in upregulation and that the two PSs (18:1/18:0, 18:0/20:5) with two PIs (18:0/18:1 and 16:1/20:2) showed similar (> 95%) downregulation. The total amount of each PL group was compared among prostate cancer patients according to the Gleason scale as larger or smaller than 6. It proposes that the current study can be utilized to sort out possible diagnostic biomarkers of prostate cancer.
English
SPRINGER HEIDELBERG
CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY
QUANTITATIVE-ANALYSIS
BREAST-CANCER
PHOSPHATIDYLCHOLINE
BRAIN
LIVER
ACID
SPECTROSCOPY
OVARIAN
TISSUE
Shotgun lipidomics for candidate biomarkers of urinary phospholipids in prostate cancer
Article
10.1007/s00216-010-4290-7
1
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.399, no.2, pp.823 - 830
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
399
2
823
830
scie
scopus
000285781900029
2-s2.0-79251627763
Biochemical Research Methods
Chemistry, Analytical
Biochemistry & Molecular Biology
Chemistry
Article
CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY
QUANTITATIVE-ANALYSIS
BREAST-CANCER
PHOSPHATIDYLCHOLINE
BRAIN
LIVER
ACID
SPECTROSCOPY
OVARIAN
TISSUE
Phospholipids
Quantitative analysis
nLC-ESI-MS/MS
Urine
Prostate cancer
Biomarker