Kim, Yeon Seok Kim, Joong Hyun Kim, In Ae Lee, Su Jin Jurng, Jongsoo Gu, Man Bock 2024-01-20T18:02:29Z 2024-01-20T18:02:29Z 2021-09-04 2010-12-15 0956-5663 https://pubs.kist.re.kr/handle/201004/130827 We have successfully developed a novel colorimetric aptasensor using gold nanoparticles for highly sensitive and specific detection of oxytetracycline (OTC), one of the most common antibacterial agents. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). Aggregation of AuNPs was specifically induced by desorption of the OTC binding aptamers (OBAs) from the surface of gold nanoparticles as a result of the aptamer-target interaction, leading to the color change from red to purple. The detection limit of OTC was enhanced up to 25 nM, which is 20-fold lower than the limit USA-EPA regulated, with two orders of magnitudes in its linear dynamic range by successful optimization on the amount of the aptamers. AuNPs, and salts. This colorimetric aptasensor is advantageous over the other conventional methods in terms of its simple signal generation and detection with the naked eye, which can be realized in on-site detection of antibacterial agents. (C) 2010 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY APTAMERS ANTIBIOTICS BIOSENSORS BINDING DESIGN A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline Article 10.1016/j.bios.2010.08.046 1 BIOSENSORS & BIOELECTRONICS, v.26, no.4, pp.1644 - 1649 BIOSENSORS & BIOELECTRONICS 26 4 1644 1649 scie scopus 000286403400077 2-s2.0-78649748231 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article; Proceedings Paper APTAMERS ANTIBIOTICS BIOSENSORS BINDING DESIGN Colorimetric detection Gold nanoparticles Aptamers Oxytetracyclines