Shen, ChangZhe Jun, HongYoung Choi, SungHo Kim, YoungMan Jung, EunJoo Oh, GiSu Joo, SungJin Kim, SungHyun Kim, IlKwang 2024-01-20T18:02:39Z 2024-01-20T18:02:39Z 2022-01-25 2010-12 0253-2964 https://pubs.kist.re.kr/handle/201004/130833 Black pine barks from the southern region of Korea were extracted using pressurized hot water and the water soluble extracts were then separated in a stepwise fashion using a variety of solvents, column chromatography (CC), thin layer chromatography (TLC), and high pressure liquid chromatography (HPLC). The antioxidant activities of each fraction and the active compounds were determined based on the radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), reductive potential of ferric ion, and total phenol contents. A DPPH test showed that the half maximal effective concentration (EC50 value : 6.59 +/- 0.31 mu g/mL) of the ethyl acetate fraction (ca. 0.67%) was almost the same as that of the control compounds and inversely proportional to the value of the total phenol contents. The cell viability of the water extracts was confirmed by methyl thiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) with enzyme linked immune sorbent assay (ELISA). Catechin, epicatechin, quercetin and ferulic acid were isolated from the ethyl acetate fraction as active compounds and identified by nuclear magnetic resonance. The antioxidant activity as value of DPPH of each of the separated compounds was lower than the ethyl acetate fraction, and ferulic acid was the lowest among these compounds. English WILEY-V C H VERLAG GMBH Evaluation of Antioxidant Activities and Active Compounds Separated from Water Soluble Extracts of Korean Black Pine Barks Article 10.5012/bkcs.2010.31.12.3567 1 BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.31, no.12, pp.3567 - 3572 BULLETIN OF THE KOREAN CHEMICAL SOCIETY 31 12 3567 3572 N scie scopus kci 000286150900016 2-s2.0-78650340317 Chemistry, Multidisciplinary Chemistry Article IN-VITRO METABOLISM PYCNOGENOL(R) QUERCETIN HYDROXYL NEEDLES ACID Black pine bark Antioxidant activity Total phenol content Reductive potential DPPH