Lim, Young Chang Cho, K. Woong Kwon, Hak Cheol Kang, Sung Un Pyun, Jung Hee Lee, Mi Hye Hwang, Hye Sook Kim, Jang Hee Lee, Ha Neul Choi, Eun Chang Kim, Chul-Ho 2024-01-20T18:02:44Z 2024-01-20T18:02:44Z 2022-01-25 2010-12 1976-8710 https://pubs.kist.re.kr/handle/201004/130838 Objectives. To determine whether a novel marine micro-organism with anticancer properties, H31, the metabolic product of Bacillus SW31, has anti-tumor effects on head and neck cancer, and potential for apoptotic-enhancing anti-cancer treatment of affected patients. Methods. The cell viability and apoptosis assays were performed. Changes in the signal pathway related to apoptosis were investigated. Then, the therapeutic effects of H31 were explored in mouse xenograft model and drug toxicity of H31. was examined in zebrafish model. Results. We identified the anticancer activity of H31, a novel metabolic product of Bacillus SW31. Bacillus SW31, a new marine micro-organism, has 70% homology with Bacillus firms and contains potent cytotoxic bioactivity in head and neck cancer cells using MU assay. Combined with c-JUN, p53, cytochrome C, and caspase-3, H31 induced apoptosis of KB cells, a head and neck cancer cell line. In a separate in vivo model, tumor growth in C3H/HeJ syngeneic mice was suppressed by H31. In addition, in a zebrafish model used for toxicity testing, a considerable dose of H31 did not result in embryo or neurotoxicity. Conclusion. Growth inhibition and apoptosis were achieved both in vitro and in vivo in head and neck cancer cells after exposure to H31, a metabolite from the marine Bacillus species, without any significant toxicity effects even at considerable H31 dose concentrations. English KOREAN SOC OTORHINOLARYNGOL Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells Article 10.3342/ceo.2010.3.4.217 1 CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, v.3, no.4, pp.217 - 225 CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY 3 4 217 225 N scie scopus kci other 000285863100008 2-s2.0-78650839496 Otorhinolaryngology Otorhinolaryngology Article COMBINATORIAL APPROACH NATURAL-PRODUCTS CARCINOMA-CELLS CHEMOTHERAPY SPECIFICITIES ZEBRAFISH NOV P53 Apoptosis Bacillus Marine toxins Head and neck cancer Cytotoxicity