You, Dong-Joo Kim, You Lim Park, Cho Rong Kim, Dong-Kyu Yeom, Jeonghun Lee, Cheolju Ahn, Curie Seong, Jae Young Hwang, Jong-Ik 2024-01-20T18:03:11Z 2024-01-20T18:03:11Z 2021-09-05 2010-12 1016-8478 https://pubs.kist.re.kr/handle/201004/130859 G protein beta-like (G beta L) is a member of WD repeat-containing family which are involved in various intracellular signaling events. In our previous report, we demonstrated that G beta L regulates TNF alpha-stimulated NF-kappa B signaling by interacting with and inhibiting phosphorylation of I kappa B kinase. However, G beta L itself does not seem to regulate IKK directly, because it contains no functional domains except WD domains. Here, using immunoprecipitation and proteomic analyses, we identified protein phosphatase 4 as a new binding partner of G beta L. We also found that G beta L interacts with PP2A and PP6, other members of the same phosphatase family. By interacting with protein phosphatases, which do not directly bind to IKK beta, G beta L mediates the association of phosphatases with IKK beta. Overexpression of protein phosphatases inhibited TNF kappa-induced activation of NF-kappa B signaling, which is an effect similar to that of G beta L overexpression. Down-regulation of G beta L by small interfering RNA diminished the inhibitory effect of phosphatases, resulting in restoration of NF-kappa B signaling. Thus, we propose that G beta L functions as a negative regulator of NF-kappa B signaling by recruiting protein phosphatases to the IKK complex. English KOREAN SOC MOLECULAR & CELLULAR BIOLOGY INSULIN-RECEPTOR SUBSTRATE-1 WD-REPEAT ACTIVATION NEMO IKK PHOSPHORYLATION PATHWAY UBIQUITINATION DISEASES COMPLEX Regulation of I kappa B kinase by G beta L through recruitment of the protein phosphatases Article 10.1007/s10059-010-0155-3 1 MOLECULES AND CELLS, v.30, no.6, pp.527 - 532 MOLECULES AND CELLS 30 6 527 532 scie scopus kci ART001512581 000287596000005 2-s2.0-79957747120 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article INSULIN-RECEPTOR SUBSTRATE-1 WD-REPEAT ACTIVATION NEMO IKK PHOSPHORYLATION PATHWAY UBIQUITINATION DISEASES COMPLEX G beta L I kappa B kinase NF-kappa B phosphorylation protein phosphatases