Choi, Mina Cho, Minjung Han, Beom Seok Hong, Jin Jeong, Jayoung Park, Sangjin Cho, Myung-Haing Kim, Kwangmeyung Cho, Wan-Seob 2024-01-20T18:04:08Z 2024-01-20T18:04:08Z 2021-09-04 2010-11-30 0378-4274 https://pubs.kist.re.kr/handle/201004/130904 Pulmonary delivery of nanoparticles (NP) conjugated with therapeutic agents has been considered recently for both lung disorders and systemic circulation. Hydrophobically modified glycol chitosan (HGC) NP have previously shown excellent deposition to the tumor site and non-destructive intracellular release. Here, we evaluated the kinetics and toxicity of HGC NP by intratracheal instillation to mice. HGC NP showed a positive charge and average hydrodynamic size was around 350 nm. The half-life of NP in the lung was determined as 131.97+/-50.51 h. NP showed rapid uptake into systemic circulation and excretion via urine which was peaked at 6 h after instillation. Although HGC NP were distributed to several extrapulmonary organs, the levels were extremely low and transient. HGC NP induced transient neutrophilic pulmonary inflammation from 6 h to day 3 after instillation. Expression of pro-inflammatory cytokines (IL-1 beta, IL-6, and TNF-alpha) and chemokine (MIP-1 alpha) in lung showed an increase from 1 h to 24 h after instillation and recovered thereafter. Our findings suggest that HGC NP can be successful candidates for use as pulmonary delivery vehicles, owing to their excellent biocompatibility, transiency, and low pulmonary toxicity, and property of rapid elimination without accumulation. Crown Copyright (C) 2010 Published by Elsevier Ireland Ltd. All rights reserved. English ELSEVIER IRELAND LTD SELF-ASSEMBLED NANOPARTICLES DRUG-DELIVERY INTRATRACHEAL INSTILLATION OXIDE NANOPARTICLES GOLD NANOPARTICLES IN-VITRO PARTICLES TOXICITY INSULIN BIODISTRIBUTION Chitosan nanoparticles show rapid extrapulmonary tissue distribution and excretion with mild pulmonary inflammation to mice Article 10.1016/j.toxlet.2010.08.016 1 TOXICOLOGY LETTERS, v.199, no.2, pp.144 - 152 TOXICOLOGY LETTERS 199 2 144 152 scie scopus 000284493800006 2-s2.0-77957951953 Toxicology Toxicology Article SELF-ASSEMBLED NANOPARTICLES DRUG-DELIVERY INTRATRACHEAL INSTILLATION OXIDE NANOPARTICLES GOLD NANOPARTICLES IN-VITRO PARTICLES TOXICITY INSULIN BIODISTRIBUTION Hydrophobically modified glycol chitosan Pulmonary delivery vehicle Intratracheal instillation Tissue distribution Elimination