Young, Eric Lee, Sun-Mi Alper, Hal 2024-01-20T18:04:28Z 2024-01-20T18:04:28Z 2021-09-05 2010-11-16 1754-6834 https://pubs.kist.re.kr/handle/201004/130919 Hexose and pentose cofermentation is regarded as one of the chief obstacles impeding economical conversion of lignocellulosic biomass to biofuels. Over time, successful application of traditional metabolic engineering strategy has produced yeast strains capable of utilizing the pentose sugars (especially xylose and arabinose) as sole carbon sources, yet major difficulties still remain for engineering simultaneous, exogenous sugar metabolism. Beyond catabolic pathways, the focus must shift towards non-traditional aspects of cellular engineering such as host molecular transport capability, catabolite sensing and stress response mechanisms. This review highlights the need for an approach termed 'panmetabolic engineering', a new paradigm for integrating new carbon sources into host metabolic pathways. This approach will concurrently optimize the interdependent processes of transport and metabolism using novel combinatorial techniques and global cellular engineering. As a result, panmetabolic engineering is a whole pathway approach emphasizing better pathways, reduced glucose-induced repression and increased product tolerance. In this paper, recent publications are reviewed in light of this approach and their potential to expand metabolic engineering tools. Collectively, traditional approaches and panmetabolic engineering enable the reprogramming of extant biological complexity and incorporation of exogenous carbon catabolism. English BIOMED CENTRAL LTD RECOMBINANT SACCHAROMYCES-CEREVISIAE PICHIA-STIPITIS L-ARABINOSE XYLOSE-ISOMERASE ETHANOL-PRODUCTION ALCOHOLIC FERMENTATION HEXOSE TRANSPORTERS BIOFUELS PRODUCTION CANDIDA-INTERMEDIA ESCHERICHIA-COLI Optimizing pentose utilization in yeast: the need for novel tools and approaches Article 10.1186/1754-6834-3-24 1 BIOTECHNOLOGY FOR BIOFUELS, v.3 BIOTECHNOLOGY FOR BIOFUELS 3 scie scopus 000284687200001 2-s2.0-78649922301 Biotechnology & Applied Microbiology Energy & Fuels Biotechnology & Applied Microbiology Energy & Fuels Review RECOMBINANT SACCHAROMYCES-CEREVISIAE PICHIA-STIPITIS L-ARABINOSE XYLOSE-ISOMERASE ETHANOL-PRODUCTION ALCOHOLIC FERMENTATION HEXOSE TRANSPORTERS BIOFUELS PRODUCTION CANDIDA-INTERMEDIA ESCHERICHIA-COLI