Jeon, Byoung Seung Kim, Byung-Chun Um, Youngsoon Sang, Byoung-In 2024-01-20T18:30:34Z 2024-01-20T18:30:34Z 2021-09-04 2010-11 0175-7598 https://pubs.kist.re.kr/handle/201004/130975 In a study screening anaerobic microbes utilizing d-galactitol as a fermentable carbon source, four bacterial strains were isolated from an enrichment culture producing H-2, ethanol, butanol, acetic acid, butyric acid, and hexanoic acid. Among these isolates, strain BS-1 produced hexanoic acid as a major metabolic product of anaerobic fermentation with d-galactitol. Strain BS-1 belonged to the genus Clostridium based on phylogenetic analysis using 16S rRNA gene sequences, and the most closely related strain was Clostridium sporosphaeroides DSM 1294(T), with 94.4% 16S rRNA gene similarity. In batch cultures, Clostridium sp. BS-1 produced 550 +/- 31 mL L-1 of H-2, 0.36 +/- 0.01 g L-1 of acetic acid, 0.44 +/- 0.01 g L-1 of butyric acid, and 0.98 +/- 0.03 g L-1 of hexanoic acid in a 4-day cultivation. The production of hexanoic acid increased to 1.22 and 1.73 g L-1 with the addition of 1.5 g L-1 of sodium acetate and 100 mM 2-(N-morpholino)ethanesulfonic acid (MES), respectively. Especially when 1.5 g L-1 of sodium acetate and 100 mM MES were added simultaneously, the production of hexanoic acid increased up to 2.99 g L-1. Without adding sodium acetate, 2.75 g L-1 of hexanoic acid production from d-galactitol was achieved using a coculture of Clostridium sp. BS-1 and one of the isolates, Clostridium sp. BS-7, in the presence of 100 mM MES. In addition, volatile fatty acid (VFA) production by Clostridium sp. BS-1 from d-galactitol and d-glucose was enhanced when a more reduced culture redox potential (CRP) was applied via addition of Na2S center dot 9H(2)O. English SPRINGER Production of hexanoic acid from D-galactitol by a newly isolated Clostridium sp. BS-1 Article 10.1007/s00253-010-2827-5 1 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.88, no.5, pp.1161 - 1167 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 88 5 1161 1167 N scie scopus 000283095500013 2-s2.0-78149399843 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article HYDROGEN-PRODUCTION MEGASPHAERA-ELSDENII SEQUENCE ALIGNMENT CAPROIC ACID BUTYRIC-ACID ACETOBUTYLICUM METABOLISM KLUYVERI STRAINS Clostridium sp. BS-1 Sludge Hexanoic acid D-galactitol Coculture