Choi, Dong Hoon Park, Chul Ho Kim, Ik Hwan Chun, Heung Jae Park, Kwideok Han, Dong Keun 2024-01-20T18:31:09Z 2024-01-20T18:31:09Z 2021-09-04 2010-10-15 0168-3659 https://pubs.kist.re.kr/handle/201004/131004 To effectively harness the great potential of stem cells, we designed a dual growth factor delivery system for the application toward stem cell differentiation into specific lineages. This system carries a core shell structure within microcapsules made of poly(L-lactide-co-glycolide) (PLGA) and alginate, which were fabricated using a coaxial electro-dropping method. Both PLGA and alginate were supplied from the inner and outer nozzles, respectively. The size and shape of microcapsules were greatly varying depending on the variables: nozzle size, applied voltage, volumetric feeding ratio (PLGA:alginate), feeding rate, and polymer concentrations. Once proper conditions were met, single or multi PLGA cores were found settled within the microcapsules. From the microscopic images, wrinkled surfaces of microcapsules were observed, along with the PLGA cores inside the alginate domain. When two different microcapsules were made, switching the position of bone morphogenetic protein (BMP)-2 and dexamethasone (Dex) for either core or shell domain, their release profiles were very unique on a temporal basis, based on their location in the microcapsules. An initial burst of biomolecules was highly suppressed when either biomolecule was loaded in the PLGA core. It was clear that the osteogenic biomolecules encapsulated in the microcapsule could be released together and their concentrations were disparate at each time point. Meanwhile as the hydrogel constructs including rat bone marrow stromal cells (BMSCs) and osteogenic factor-loaded microcapsules were cultured for up to 4 weeks, the gene expressions levels of osteopontin, type I collagen, and osteocalcin were significantly upregulated as compared to the control group. The present coaxial system was very effective in manufacturing PLGA core alginate shell microcapsules and in encapsulating multiple biomolecules essential for stem cell differentiation. (C) 2010 Elsevier B.V. All rights reserved. English ELSEVIER CHONDROGENIC DIFFERENTIATION CONTROLLED-RELEASE CROSS-LINKING MICROSPHERES GENIPIN Fabrication of core-shell microcapsules using PLGA and alginate for dual growth factor delivery system Article 10.1016/j.jconrel.2010.07.103 1 JOURNAL OF CONTROLLED RELEASE, v.147, no.2, pp.193 - 201 JOURNAL OF CONTROLLED RELEASE 147 2 193 201 scie scopus 000283566600007 2-s2.0-77957018566 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article CHONDROGENIC DIFFERENTIATION CONTROLLED-RELEASE CROSS-LINKING MICROSPHERES GENIPIN Dual growth factor delivery Coaxial system Core-shell microcapsule PLGA Alginate Stem cells