Kang, Kyungsu Jin, Yong-Mei Jeon, Hyesung Park, Sang-Ryoung Song, Dae-geun Lee, Joo Young Pan, Cheol-Ho Kim, Minkyun 2024-01-20T18:32:05Z 2024-01-20T18:32:05Z 2021-09-05 2010-10 1863-5466 https://pubs.kist.re.kr/handle/201004/131048 Multiple sequence alignments showed that the prolines at the 25th, 129th, 153rd, 242nd, 322nd, and 434th amino acids in 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Agrobacterium sp. strain CP4 are strongly conserved in various prokaryotic EPSPS proteins. Single point mutations of the conserved prolines to alanine (P25A, P153A, P242A, P322A, and P434A) were introduced in the CP4 EPSPS in order to investigate the importance of the conserved prolines for the enzyme properties. The point mutations caused decreases in substrate binding affinity and catalytic efficiency as well as the glyphosate resistance, in general. Especially, the 25th and 242nd prolines located in the polypeptide hinges connecting top and bottom domains of CP4 EPSPS as well as the 434th proline at the C-terminus of the enzyme turned out to be crucial for the enzyme activity. English SPRINGER GLYPHOSATE HERBICIDE BIOSYNTHESIS INHIBITION PNEUMONIAE The three proline residues (P25, P242, and P434) of Agrobacterium CP4 5-enolpyruvylshikimate-3-phosphate synthase are crucial for the enzyme activity Article 10.1007/s11816-010-0150-3 1 PLANT BIOTECHNOLOGY REPORTS, v.4, no.4, pp.329 - 334 PLANT BIOTECHNOLOGY REPORTS 4 4 329 334 scie scopus kci other ART001495410 000282871900010 2-s2.0-77958453863 Biotechnology & Applied Microbiology Plant Sciences Biotechnology & Applied Microbiology Plant Sciences Article GLYPHOSATE HERBICIDE BIOSYNTHESIS INHIBITION PNEUMONIAE Agrobacterium sp strain CP4 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) Glyphosate Herbicide resistance Proline residue