Kwon, Ki Young Youn, Jongkyu Kim, Jae Hyun Park, Yongjin Jeon, Chulmin Kim, Byoung Chan Kwon, Yongchai Zhao, Xueyan Wang, Ping Sang, Byoung In Lee, Jinwoo Park, Hyun Gyu Chang, Ho Nam Hyeon, Taeghwan Ha, Su Jung, Hee-Tae Kim, Jungbae 2024-01-20T18:32:18Z 2024-01-20T18:32:18Z 2021-09-05 2010-10 0956-5663 https://pubs.kist.re.kr/handle/201004/131059 Nanoscale enzyme reactors (NERs) of glucose oxidase in conductive mesoporous carbons were prepared in a two-step process of enzyme adsorption and follow-up enzyme crosslinking. MSU-F-C, a mesoprous carbon, has a bottleneck pore structure with mesocellular pores of 26 nm connected with window mesopores of 17 nm. This structure enables the ship-in-a-bottle mechanism of NERs, which effectively prevents the crosslinked enzymes in mesocellular pores from leaching through the smaller window mesopores. This NER approach not only stabilized the enzyme but also expedited electron transfer between the enzyme and the conductive MSU-F-C by maintaining a short distance between them. In a comparative study with GOx that was simply adsorbed without crosslinking, the NER approach was proven to be effective in improving the sensitivity of glucose biosensors and the power density of biofuel cells. The power density of biofuel cells could be further improved by manipulating several factors, such as by adding a mediator, changing the order of adsorption and crosslinking, and inserting a gold mesh as an electron collector. (C) 2010 Elsevier B.V. All rights reserved. English Pergamon Press Ltd. Nanoscale enzyme reactors in mesoporous carbon for improved performance and lifetime of biosensors and biofuel cells Article 10.1016/j.bios.2010.07.001 1 Biosensors and Bioelectronics, v.26, no.2, pp.655 - 660 Biosensors and Bioelectronics 26 2 655 660 N scie scopus 000283804400057 2-s2.0-77956902537 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article ORGANIC-SOLVENTS SILICA STABILIZATION BIOCATALYSIS STABILITY FOAM Biofuel cells Biosensors Enzyme crosslinking Mesoporous carbon Nanoscale enzyme reactors