Hyeon, Jeong-eun Yu, Kyung-Ok Suh, Dong Jin Suh, Young-Woong Lee, Sung Eun Lee, Jinwon Han, Sung Ok 2024-01-20T18:34:25Z 2024-01-20T18:34:25Z 2021-09-04 2010-09 0378-1097 https://pubs.kist.re.kr/handle/201004/131156 Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl-cellulose (CMC)] into ethanol with the aid of beta-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose-binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L-1 initial CMC, approximately 3.45 g L-1 ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g-1 from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes. English WILEY-BLACKWELL CELL-SURFACE AMORPHOUS CELLULOSE CRYSTALLINE CELLULOSE TRICHODERMA-REESEI BACILLUS-SUBTILIS BETA-GLUCOSIDASE DEGRADATION ENZYMES YEAST EXPRESSION Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae Article 10.1111/j.1574-6968.2010.02035.x 1 FEMS MICROBIOLOGY LETTERS, v.310, no.1, pp.39 - 47 FEMS MICROBIOLOGY LETTERS 310 1 39 47 scie scopus 000280633200006 2-s2.0-77955266119 Microbiology Microbiology Article CELL-SURFACE AMORPHOUS CELLULOSE CRYSTALLINE CELLULOSE TRICHODERMA-REESEI BACILLUS-SUBTILIS BETA-GLUCOSIDASE DEGRADATION ENZYMES YEAST EXPRESSION minicellulosome Clostridium cellulovorans Saccharomyces cerevisiae cellulose-binding domain consolidated bioprocessing ethanol fermentation