Choi, Soonwook Yu, Eunah Kim, Daesoo Urbano, Francisco J. Makarenko, Vladimir Shin, Hee-Sup Llinas, Rodolfo R. 2024-01-20T18:34:48Z 2024-01-20T18:34:48Z 2021-09-05 2010-08-15 0022-3751 https://pubs.kist.re.kr/handle/201004/131174 The role of P/Q- and T-type calcium channels in the rhythmic oscillatory behaviour of inferior olive (IO) neurons was investigated in mutant mice. Mice lacking either the Ca(V)2.1 gene of the pore-forming alpha 1A subunit for P/Q-type calcium channel, or the Ca(V)3.1 gene of the pore-forming alpha 1G subunit for T-type calcium channel were used. In vitro intracellular recording from IO neurons reveals that the amplitude and frequency of sinusoidal subthreshold oscillations (SSTOs) were reduced in the Ca(V)2.1-/- mice. In the Ca(V)3.1-/- mice, IO neurons also showed altered patterns of SSTOs and the probability of SSTO generation was significantly lower (15%, 5 of 34 neurons) than that of wild-type (78%, 31 of 40 neurons) or Ca(V)2.1-/- mice (73%, 22 of 30 neurons). In addition, the low-threshold calcium spike and the sustained endogenous oscillation following rebound potentials were absent in IO neurons from Ca(V)3.1-/- mice. Moreover, the phase-reset dynamics of oscillatory properties of single neurons and neuronal clusters in IO were remarkably altered in both Ca(V)2.1-/- and Ca(V)3.1-/- mice. These results suggest that both alpha 1A P/Q- and alpha 1G T-type calcium channels are required for the dynamic control of neuronal oscillations in the IO. These findings were supported by results from a mathematical IO neuronal model that incorporated T and P/Q channel kinetics. English WILEY IN-VITRO STOCHASTIC RESONANCE CEREBELLAR CAT COMMUNICATION INFORMATION MODULATION BEHAVIOR NUCLEUS BIOLOGY Subthreshold membrane potential oscillations in inferior olive neurons are dynamically regulated by P/Q- and T-type calcium channels: a study in mutant mice Article 10.1113/jphysiol.2009.184705 1 JOURNAL OF PHYSIOLOGY-LONDON, v.588, no.16, pp.3031 - 3043 JOURNAL OF PHYSIOLOGY-LONDON 588 16 3031 3043 scie scopus 000281001900007 2-s2.0-77955753406 Neurosciences Physiology Neurosciences & Neurology Physiology Article IN-VITRO STOCHASTIC RESONANCE CEREBELLAR CAT COMMUNICATION INFORMATION MODULATION BEHAVIOR NUCLEUS BIOLOGY