Park, Hye-Won Jung, Hana Choi, Kee-Hyun Baik, Ja-Hyun Rhim, Hyewhon 2024-01-20T18:34:59Z 2024-01-20T18:34:59Z 2021-09-05 2010-08-04 0014-5793 https://pubs.kist.re.kr/handle/201004/131183 Although Ca(v)1.2 and Ca(v)1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of Ca(v)1.3 have not been well elucidated compared to Ca(v)1.2. Here, we found that Ca(v)1.3-NT associates with GABA(B)R2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of Ca(v)1.3 and GABA(B)R2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABA(B)R increases Ca(v)1.3 currents and intracellular Ca2+ via Ca(v)1.3, but not Ca(v)1.2. These results show a physical and functional interaction between Ca(v)1.3 and GABA(B)R, suggesting the potential pivotal roles of Ca(v)1.3 in the CNS. Structured summary: MINT-7975667: Ca(v)1.3 (uniprotkb:P27732) physically interacts (MI:0915) with GABA(B)R2 (uniprotkb:088871) by two hybrid (MI:0018) MINT-7975740: Ca(v)1.3 (uniprotkb:P27732) and GABA(B)R2 (uniprotkb:075899) colocalize (MI:0403) by fluorescence microscopy (MI:0416) MINT-7966007, MINT-7966016: Ca(v)1.3 (uniprotkb:P27732) physically interacts (MI :0915) with GABA(B)R2 (uniprotkb:088871) by anti bait coimmunoprecipitation (MI:0006) MINT-7975712, MINT-7975691: Ca(v)1.3 (uniprotkb:P27732) physically interacts (MI:0915) with GABA(B)R2 (uniprotkb:088871) by pull down (MI:0096) MINT-7966026: GABA(B)R2 (uniprotkb:088871) and Ca(v)1.3 (uniprotkb:P27732) colocalize (MI:0403) by fluorescence microscopy (MI:0416) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV FACILITATE L-TYPE CALCIUM-CHANNELS ACTIVATE NUCLEUS NEURONS Direct interaction and functional coupling between voltage-gated Ca(v)1.3 Ca2+ channel and GABA(B) receptor subunit 2 Article 10.1016/j.febslet.2010.07.014 1 FEBS LETTERS, v.584, no.15, pp.3317 - 3322 FEBS LETTERS 584 15 3317 3322 scie scopus 000281033600013 2-s2.0-77955273299 Biochemistry & Molecular Biology Biophysics Cell Biology Biochemistry & Molecular Biology Biophysics Cell Biology Article FACILITATE L-TYPE CALCIUM-CHANNELS ACTIVATE NUCLEUS NEURONS L-Type Ca2+ channels Baclofen Whole-cell patch-clamp Intracellular Ca2+