Noh, Sang Myoung Park, Myung Ok Shim, Gayong Han, Su Eun Lee, Han Young Huh, Jun Hyuk Kim, Myoung Suk Choi, Jin Joo Kim, Kwangmeyung Kwon, Ick Chan Kim, Jin-Seok Baek, Kwang-Hyun Oh, Yu-Kyoung 2024-01-20T19:01:24Z 2024-01-20T19:01:24Z 2021-09-05 2010-07-14 0168-3659 https://pubs.kist.re.kr/handle/201004/131258 For delivery of siRNA, chitosan (CS) was derivatized with poly-L-arginine (PLR) and polyethylene glycol (PEG). The formation of polyplexes with siRNA was confirmed by gel retardation. The PLR-grafted CS formed nanosized particles with siRNA. PLR-grafted CS showed higher cellular delivery efficiency of siRNA than did CS. pegylated CS, PLR, or pegylated PLR. The extent of reduction in the expression of fluorescent proteins was highest following treatment of the cells using PLR derivatives of CS in complexes with specific siRNAs. Cell viability was greater in populations treated with pegylated CS-PLR than in those treated with PLR. Hemolysis of erythrocytes was reduced upon conjugation of PLR with CS. The delivery of siRNAs via pegylated CS-PLR revealed little dependence on serum. Molecular imaging techniques revealed that the intratumoral administration of red fluorescent protein-specific siRNA in complexes with pegylated CS-PLR significantly silenced the expression of red fluorescent proteins in tumor tissues in vivo. These results indicate that pegylated CS-PLR might be useful for in vivo delivery of therapeutic siRNAs. (C) 2010 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV NUCLEIC-ACIDS IN-VITRO POLYETHYLENIMINE DNA TRANSFECTION BINDING Pegylated poly-L-arginine derivatives of chitosan for effective delivery of siRNA Article 10.1016/j.jconrel.2010.04.005 1 JOURNAL OF CONTROLLED RELEASE, v.145, no.2, pp.159 - 164 JOURNAL OF CONTROLLED RELEASE 145 2 159 164 scie scopus 000280025600011 2-s2.0-77953960004 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article NUCLEIC-ACIDS IN-VITRO POLYETHYLENIMINE DNA TRANSFECTION BINDING Chitosan Polyarginine Polyethylene glycol siRNA Serum stability