Zeng, Ke-Wu Wang, Xue-Mei Ko, Hyeonseok Yang, Hyun Ok 2024-01-20T19:01:30Z 2024-01-20T19:01:30Z 2021-09-04 2010-07-06 0378-8741 https://pubs.kist.re.kr/handle/201004/131263 Aim of the study: To investigate the neuroprotective effect of aqueous extract of modified Wu-Zi-Yan-Zong granule (MWG), a traditional Chinese herbal medicine, against CoCl2-induced neurotoxicity in PC12 cells. Materials and methods: Cell viability assay, apoptosis rate assay, ROS detection and mitochondrial membrane potential (MMP) assay were performed. In addition, cytochrome c, caspase-3. PARP and MAPKs were also detected by Western blotting. Results: MWG extract increased viability and suppresses early and middle/late stage apoptosis in a dose-dependent manner in CoCl2-induced PC12 cells. Moreover, MWG extract decreased the level of intracellular reactive oxygen species (ROS), increased MMP, regulated Bcl-2 family protein expression (Bcl-2 and Bcl-XL) and inhibited the release of cytochrome c from the mitochondria. In addition, MWG extract attenuated activation of caspase-3 and poly ADP-ribose polymerase (PARP) and inhibited the phosphorylation of ERK, c-Jun NH2-terminal kinase (JNK) and p38 MAPKs. Conclusions: MWG extract exhibited significant neuroprotective effect on PC12 cells, and this effect may be associated with the suppression of ROS generation and inhibition of mitochondria-mediated caspase and MAPK signaling pathways. (C) 2010 Elsevier Ireland Ltd. All rights reserved. English ELSEVIER IRELAND LTD ACTIVATED PROTEIN-KINASE APOPTOSIS DEATH INVOLVEMENT CASPASE-3 Neuroprotective effect of modified Wu-Zi-Yan-Zong granule, a traditional Chinese herbal medicine, on CoCl2-induced PC12 cells Article 10.1016/j.jep.2010.03.018 1 JOURNAL OF ETHNOPHARMACOLOGY, v.130, no.1, pp.13 - 18 JOURNAL OF ETHNOPHARMACOLOGY 130 1 13 18 scie scopus 000279886900003 2-s2.0-77953812776 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Pharmacology & Pharmacy Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article ACTIVATED PROTEIN-KINASE APOPTOSIS DEATH INVOLVEMENT CASPASE-3 Neuroprotection Modified Wu-Zi-Yan-Zong granule Traditional Chinese medicine PC12 cells Cobalt chloride