Liu, Chang Park, Hyun M. Monsalve, Maria V. Chen, David D. Y. 2024-01-20T19:02:18Z 2024-01-20T19:02:18Z 2021-09-02 2010-07 0022-1198 https://pubs.kist.re.kr/handle/201004/131303 Adipocere is a postmortem decomposition product consisting of mostly a mixture of free fatty acids (FFAs) that are formed because of the hydrolysis of triglycerides in adipose tissues. This article describes a simple and robust method for the extraction, identification, and quantification of FFA commonly found in adipocere using gas chromatography-mass spectrometry (GC/MS). This method was applied to analyze tissues from Kwaday Dan Ts'inchi, ancient remains discovered in a retreating glacier in the Tatshenshini-Alsek Park, British Columbia, Canada in August 1999. The lyophilized tissues were grinded and extracted with hexane. The trimethylsilyl fatty acid derivatives were analyzed by GC/MS, and the relative abundances of myristic acid, palmitic acid, oleic acid, and stearic acid were determined. Milligram per gram levels of saturated fatty acids were found in the tissues of the ancient remains, while the levels of unsaturated fatty acids, such as palmitoleic acid, were found to be negligible. The results provided further evidence of the existence of adipocere found during forensic examination of the Kwaday Dan Ts'inchi ancient remains. English WILEY BRIEF COMMUNICATION BODY PRESERVATION CANADA Free Fatty Acids Composition in Adipocere of the Kwaday Dan Ts'inchi Ancient Remains Found in a Glacier Article 10.1111/j.1556-4029.2010.01373.x 1 JOURNAL OF FORENSIC SCIENCES, v.55, no.4, pp.1039 - 1043 JOURNAL OF FORENSIC SCIENCES 55 4 1039 1043 scie scopus 000279448200027 2-s2.0-77954344209 Medicine, Legal Legal Medicine Article BRIEF COMMUNICATION BODY PRESERVATION CANADA forensic science free fatty acids adipocere extraction method gas chromatography-mass spectrometry ancient human remains