Kim, Youn-Jung Yang, Sung Ik Ryu, Jae-Chun 2024-01-20T19:02:37Z 2024-01-20T19:02:37Z 2021-09-01 2010-06-30 1738-642X https://pubs.kist.re.kr/handle/201004/131318 Nano-silver (Ag) with antimicrobial activity is by far the most commercialized nano-compound. The hazards associated with human exposure to nanosized-silver should be investigated to facilitate the risk assessment process. Recent studies have shown that inflammatory, oxidative, genotoxic, and cytotoxic consequences are associated with silver particulate exposure, and are inherently linked. In the present study, the cytotoxicity and genotoxicity of nano-silver were investigated using the dye exclusion assay, the comet assay, and the mouse lymphoma thymidine kinase (tk(+/-)) gene mutation assay (MLA). IC20 values of nano-silver in L5178Y cells were determined the concentration of 3,769.53 mu g/mL and 1,796.88 mu g/mL with and without S-9, respectively. And in BEAS-2B cell, IC20 values were calculated to 1,171.88 mu g/mL and 761.72 mu g/mL with and without S-9, respectively. From these results, nano-silver was more cytotoxic in absence of S-9 metabolic activation system and at the BEAS-2B cells. In the comet assay, L5178Y cells and BEAS-2B cells were treated with nano-silver which significantly increased > 2-fold tail moment with and without S-9. However, the mutant frequencies in the nano-silver treated L5178Y cells were slightly increased but not significant compared to the vehicle controls with and without S-9. The results of this study indicate that nano-silver can cause primary DNA damage and cytotoxicity but not mutagenicity in cultured mammalian cells. English KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT IN-VITRO TOXICITY COMET ASSAY DNA-DAMAGE NANOPARTICLES NANOSILVER APOPTOSIS IONS Cytotoxicity and genotoxicity of nano-silver in mammalian cell lines Article 10.1007/s13273-010-0018-1 1 MOLECULAR & CELLULAR TOXICOLOGY, v.6, no.2, pp.119 - 125 MOLECULAR & CELLULAR TOXICOLOGY 6 2 119 125 scie scopus kci other ART001738049 000279427900002 2-s2.0-77956565849 Biochemistry & Molecular Biology Toxicology Biochemistry & Molecular Biology Toxicology Article IN-VITRO TOXICITY COMET ASSAY DNA-DAMAGE NANOPARTICLES NANOSILVER APOPTOSIS IONS Nano-silver Cytotoxicity Comet assay Gene mutation assay (MLA) L5178Y cell BEAS-2B cell