Jung, Hyun-Jin Lee, Won-Yong Yoo, Young Sook Chung, Bong Chul Choi, Man Ho 2024-01-20T19:03:03Z 2024-01-20T19:03:03Z 2021-09-02 2010-06-03 0009-8981 https://pubs.kist.re.kr/handle/201004/131338 Background: A database-dependent gas chromatography-mass spectrometry (GC-MS) based approach was developed for non-targeted metabolite profiling, focusing on 232 steroids, 24 fatty acids, 10 eicosanoids, 10 cannabinoids and 22 steroid-fatty acid esters in biological specimens. Methods: This method, used to search for potent biomarkers in lipid metabolism, included MS based analysis combined with high-temperature gas chromatographic (HTGC) separation of biological metabolites, statistical clustering and an in-house database (DB) searching. Results: The HTGC technique showed better detectability of high lipophilic compounds, particularly steroid-fatty acid esters, which generally have poor chromatographic properties on a conventional GC column. The in-house DB search consisted of the retention index and mass spectrum corresponding to each compound selected. The method was applied to tissue samples obtained from cardiac hypertrophy-induced mice. Increased levels of palmitic, linoleic, oleic, and stearic acids and cholesterol were detected and identified. Conclusions: This data-dependent non-targeted metabolite profiling technique could be more effective in biomarker studies associated with the steroid and lipid metabolism than commercially available DBs. (C) 2010 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV LIPOTOXIC CARDIOMYOPATHY LIPID ANALYSIS METABOLOMICS URINE DISEASE CORTICOSTEROIDS HYPERTROPHY EXPRESSION ESTROGENS SYSTEMS Database-dependent metabolite profiling focused on steroid and fatty acid derivatives using high-temperature gas chromatography-mass spectrometry Article 10.1016/j.cca.2010.02.068 1 CLINICA CHIMICA ACTA, v.411, no.11-12, pp.818 - 824 CLINICA CHIMICA ACTA 411 11-12 818 824 scie scopus 000277864200007 2-s2.0-79951972384 Medical Laboratory Technology Medical Laboratory Technology Article LIPOTOXIC CARDIOMYOPATHY LIPID ANALYSIS METABOLOMICS URINE DISEASE CORTICOSTEROIDS HYPERTROPHY EXPRESSION ESTROGENS SYSTEMS Metabolite profiling Steroid Fatty acid Eicosanoids GC-MS