이주영 송대근 손진기 판철호 2024-01-20T19:03:35Z 2024-01-20T19:03:35Z 2021-09-06 2010-06 1976-0442 https://pubs.kist.re.kr/handle/201004/131364 To overcome the limitation of E. coli expression system such as inclusion body formation and disulfide bond failure, we tried to express the heterologous protein as a secreted form. We adopted agarase signal peptide (ASP; 23 amino acid residues) from Agarivorans albus YKW-34 which is one of marine bacteia. When we used ASP to express β-agarase, about 42% activity was detected in media. Korean 한국응용생명화학회 대장균에서 단백질 분비에 대한 Agarivorans albus YKW-34의 Agarase 시그널펩티드의 효과 Effect of Agarase Signal Peptide from Agarivorans albus YKW-34 on Protein Secretion in Escherichia coli Article 1 Journal of Applied Biological Chemistry, v.53, no.2, pp.105 - 107 Journal of Applied Biological Chemistry 53 2 105 107 scopus kci ART001452676 Agarivorans albus Escherichia coli recombinant protein secretion signal peptide