이장한 이용권 이지영 이승복 Kim, Sun Hwa 배귀남 이학성 임철수 정남현 2024-01-20T19:03:40Z 2024-01-20T19:03:40Z 2021-09-06 2010-06 2093-6400 https://pubs.kist.re.kr/handle/201004/131368 DEPs (diesel exhaust particles) like any other particles can be also inhaled into lung to participate in a damaging reaction to the organ. Possible damages might be apoptosis and inflammatory responses to the cells in respiratory track. The aim of this study was cytotoxicity evaluation of DEPs from five in-use diesel vehicles using a murine macrophage cell (RAW 254.7). We found that most DEPs have a considerable cytotoxicity compared to the control and SRM 2975. When measured by MTT assay and extents of apoptosis, DEPs of two highmileage vehicles had higher toxicity than those of the other three low-mileage vehicles tested. Although mRNA expression level of TNF-α somewhat explains the trend of cytotoxicity and apoptosis, that of IL-1β did not. Correlation studies among the extents of MTT assay, apoptosis, and TNF-α expression showed that the extents between apoptosis and TNF-α expression was most highly correlated (r=0.96). These results suggest that cytotoxicity of various DEPs could be compared easily by measuring the extent of apoptosis or TNF-α expression by DEPs. Korean 환경독성보건학회 국내 디젤 차량 배기 입자가 쥐 대식세포에 미치는 세포독성 평가 Cytotoxicity of Diesel Exhaust Particles from Various Vehicles toward Macrophage Cells Article 2 환경독성보건학회지, v.25, no.2, pp.111 - 120 환경독성보건학회지 25 2 111 120 N kci ART001463165 Diesel exhaust particle Macrophage Cytotoxicity Apoptosis TNF-α expression