Min Sun Lee 이영주 정봉철 정병화 2024-01-20T19:03:52Z 2024-01-20T19:03:52Z 2021-09-06 2010-06 2093-5552 https://pubs.kist.re.kr/handle/201004/131378 A gas chromatographic-mass spectrometric (GC-MS) method was developed for the simultaneous determination of valproic acid (VPA) and its toxic metabolites, 4-ene-VPA and 2,4-diene-VPA in rat plasma. Extraction was performed in weak acidic condition (pH 5.2) to avoid degradation of 4-ene-VPA and 2,4-diene-VPA. The recoveries for 4-ene-VPA and 2,4-diene-VPA were more than 70% and that for VPA was 33-42%. R value for each compounds exceeded 0.998 in calibration curve during all the analysis. Accuracy and precision ranged from 88.3 to 113.2% and from 2.16 to 14.2%,respectively The method was successfully applied to monitor plasma concentrations of VPA, 4-ene-VPA and 2,4-diene-VPA after intravenous administration of VPA at the dose of 100 mg/kg, suggesting that these toxic metabolites may involved in the hepatotoxicity induced by VPA. English 한국약제학회 Simultaneous Determination of Valproic Acid and its Toxic Metabolites, 4-ene-VPA and 2,4-diene-VPA in Rat Plasma using a Gas Chromatographic-mass Spectrometric Method Article 2 Journal of Pharmaceutical Investigation, v.40, no.3, pp.155 - 160 Journal of Pharmaceutical Investigation 40 3 155 160 kci ART001451175 valproic acid (VPA) 4-ene-VPA 2 4-diene-VPA quantitative analysis GC-MS (gas chromatographic-mass spectrometry) pharmacokinetics