Kang, Gil Bu Song, Hye-Eun Kim, Mun-Kyoung Youn, Hyung-Seop Lee, Jung-Gyu An, June Yop Chun, Jang-Soo Jeon, Hyesung Eom, Soo Hyun 2024-01-20T19:04:07Z 2024-01-20T19:04:07Z 2021-09-02 2010-06 0950-382X https://pubs.kist.re.kr/handle/201004/131392 P>In Gram-negative bacteria, proper placement of the FtsZ ring, mediated by nucleoid occlusion and the activities of the dynamic oscillating Min proteins MinC, MinD and MinE, is required for correct positioning of the cell division septum. MinE is a topological specificity factor that counters the activity of MinCD division inhibitor at the mid-cell division site. Its structure consists of an anti-MinCD domain and a topology specificity domain (TSD). Previous NMR analysis of truncated Escherichia coli MinE showed that the TSD domain contains a long alpha-helix and two anti-parallel beta-strands, which mediate formation of a homodimeric alpha/beta structure. Here we report the crystal structure of full-length Helicobacter pylori MinE and redefine its TSD based on that structure. The N-terminal region of the TSD (residues 19-26), previously defined as part of the anti-MinCD domain, forms a beta-strand (beta A) and participates in TSD folding. In addition, H. pylori MinE forms a dimer through the interaction of anti-parallel beta A-strands. Moreover, we observed serial dimer-dimer interactions within the crystal packing, resulting in the formation of a multimeric structure. We therefore redefine the functional domain of MinE and propose that a multimeric filamentous structure is formed through anti-parallel beta-strand interactions. English WILEY ESCHERICHIA-COLI PROPER PLACEMENT REGULATOR MINE SITE ATPASE LOCALIZATION OSCILLATION PROTEIN RING STIMULATION Crystal structure of Helicobacter pylori MinE, a cell division topological specificity factor Article 10.1111/j.1365-2958.2010.07160.x 1 MOLECULAR MICROBIOLOGY, v.76, no.5, pp.1222 - 1231 MOLECULAR MICROBIOLOGY 76 5 1222 1231 scie scopus 000277981100012 2-s2.0-77952838326 Biochemistry & Molecular Biology Microbiology Biochemistry & Molecular Biology Microbiology Article ESCHERICHIA-COLI PROPER PLACEMENT REGULATOR MINE SITE ATPASE LOCALIZATION OSCILLATION PROTEIN RING STIMULATION