Vendrell, Marc Lee, Jun-Seok Chang, Young-Tae 2024-01-20T19:04:56Z 2024-01-20T19:04:56Z 2021-09-01 2010-06 1367-5931 https://pubs.kist.re.kr/handle/201004/131433 Diversity-oriented fluorescence library approaches have significantly accelerated the development of new sensors. By making use of combinatorial chemistry and high-throughput screening, they can circumvent our limitations in designing probes for particular recognition processes. Combinatorial chemists have proved how to derivatize fluorogenic scaffolds, tune their photophysical spectra and adjust their properties (from cell permeability to quantum yields) to generate libraries of potential sensors. Several platforms (in vitro assays, cell-based imaging) have also been optimized to screen these libraries in a high-throughput manner, and with the recent progress in image acquisition and analysis, their scope has been expanded toward more diverse and demanding biological systems. Supported by successful examples of fluorescent sensors for biomolecules, proteins, or even phenotypes, this review (together with a video abstract) stresses the important role that diversity-oriented approaches will continue to play in probe development in the near future. English ELSEVIER SCI LTD SOLID-PHASE SYNTHESIS COMBINATORIAL DISCOVERY PARALLEL SYNTHESIS IMAGING PROBES SENSORS DYES FLUOROPHORES DERIVATIVES CHEMOSENSORS Diversity-oriented fluorescence library approaches for probe discovery and development Article 10.1016/j.cbpa.2010.02.020 1 CURRENT OPINION IN CHEMICAL BIOLOGY, v.14, no.3, pp.383 - 389 CURRENT OPINION IN CHEMICAL BIOLOGY 14 3 383 389 scie scopus 000279216500012 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Review SOLID-PHASE SYNTHESIS COMBINATORIAL DISCOVERY PARALLEL SYNTHESIS IMAGING PROBES SENSORS DYES FLUOROPHORES DERIVATIVES CHEMOSENSORS Chemical Biology Combinatorial Chemistry Fluorescence probe