Lee, Jung-Ok Chang, Kiyuk Kim, Chul Young Jung, Sang Hoon Lee, Seung-Woo Oak, Min-Ho 2024-01-20T19:30:53Z 2024-01-20T19:30:53Z 2021-09-02 2010-05 0160-2446 https://pubs.kist.re.kr/handle/201004/131483 Lysimachia clethroides is widely used in traditional herbal medicine for many purposes, especially for blood vessel-related diseases in Korea and East Asia. Experiments were undertaken to determine whether hydro-alcoholic extract obtained from L. clethroides (LCE) has vasorelaxant activity in the rat aorta rings and, if so, to elucidate the underlying mechanism. Rat aorta rings were suspended in organ chambers for the measurement of changes in isometric tension in the presence or absence of several inhibitors. LCE induced endothelium-dependent vasodilation (ED50 = 6.1 mu g/mL) and that was abolished by nitric oxide synthase inhibitor, N-omega-nitro-L-arginine, and guanylyl cyclase inhibitor, 1H-[1,2,4] oxadiazolo [4,3-a] quinoxalin-1-one, PI3-kinase inhibitor, wortmannin, and cell permeable superoxide dismutase. In addition, LCE decreased vessels contraction by phenylephrine. Prostaglandin synthesis inhibitor, indometacin, and inhibitors of endothelium-derived hyperpolarizing factor, charybdotoxin plus apamin, did not affect vasodilatory effect of LCE. In cultured endothelial cells, LCE-induced phosphorylation of serine 1177-endothelial nitric oxide synthase and serine 473-Akt. LCE inhibited strongly nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity in smooth muscle cells and angiotensin II-induced contraction of rat aorta. Finally, increased oxidative stress in rat aorta-induced by angiotensin II is ameliorated by LCE. Taken together, LCE induces an endothelium-dependent vasodilation and might be involved, at least in part, the activation of the nitric oxide-cyclic guanosine monophosphate pathway. In addition, LCE decreases oxidative stress in aorta by inhibition of NADPH oxidase activity. The present findings indicate that LCE could be a candidate of herbal medicine for cardiovascular diseases associated with disturbed NO production and endothelial dysfunction. English LIPPINCOTT WILLIAMS & WILKINS PORCINE CORONARY-ARTERIES NITRIC-OXIDE SYNTHASE EDHF-MEDIATED RELAXATIONS ANGIOTENSIN-II RED WINE SUPEROXIDE-PRODUCTION NADPH OXIDASE OXIDATIVE STRESS NAD(P)H OXIDASE ACTIVATION Lysimachia clethroides Extract Promote Vascular Relaxation via Endothelium-Dependent Mechanism Article 10.1097/FJC.0b013e3181d7066f 1 JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, v.55, no.5, pp.481 - 488 JOURNAL OF CARDIOVASCULAR PHARMACOLOGY 55 5 481 488 scie scopus 000278205900008 2-s2.0-77952975366 Cardiac & Cardiovascular Systems Pharmacology & Pharmacy Cardiovascular System & Cardiology Pharmacology & Pharmacy Article PORCINE CORONARY-ARTERIES NITRIC-OXIDE SYNTHASE EDHF-MEDIATED RELAXATIONS ANGIOTENSIN-II RED WINE SUPEROXIDE-PRODUCTION NADPH OXIDASE OXIDATIVE STRESS NAD(P)H OXIDASE ACTIVATION Lysimachia clethroides endothelium eNOS NADPH oxidase vasodilation