Lee, Yeon Sil Kim, Seon Ha Jung, Sang Hoon Kim, Jin Kyu Pan, Cheol-Ho Lim, Soon Sung 2024-01-20T19:31:12Z 2024-01-20T19:31:12Z 2021-09-02 2010-05 0918-6158 https://pubs.kist.re.kr/handle/201004/131499 We evaluated the inhibitory effects of components from the root of Glycyrrhiza uralensis (G. uralensis) on aldose reductase (AR) and sorbitol formation in rat lenses with high levels of glucose as part of our ongoing search of natural sources for therapeutic and preventive agents for diabetic complications. In order to identify the bioactive components of G. uralensis, 5 prenylated flavonoids (semilicoisoflavone B, 7-O-methylluteone, dehydroglyasperin C, dehydroglyasperin D, and isoangustone A), three flavonoids (liquiritigenin, isoliquiritigenin, and licochalcone A), and two triterpenoids (glycyrrhizin and glycyrrhetinic acid) were isolated; their chemical structures were then elucidated on the basis of spectroscopic evidence and comparison with published data. The anti-diabetic complication activities of 10 G. uralensis-derived components were investigated via inhibitory assays using rat lens AR (rAR) and human recombinant AR (rhAR). From the 10 isolated compounds, semilicoisoflavone B showed the most potent inhibition, with the IC50 values of rAR and rhAR at 1.8 and 10.6 mu M, respectively. In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/concentration of substrate, semilicoisoflavone B showed noncompetitive inhibition against rhAR. The results clearly indicated that the presence of a gamma,gamma-dimethylchromene ring is partly responsible for the AR inhibitory activity of isoprenoid-type flavonoids. Further, semilicoisoflavone B inhibited sorbitol formation of rat lens incubated with a high concentration of glucose, indicating that this compound may be effective for preventing osmotic stress in hyperglycemia. English PHARMACEUTICAL SOC JAPAN ADVANCED GLYCATION ENDPRODUCTS DIABETIC COMPLICATIONS PHENOLIC-COMPOUNDS KK-A(Y) MICE LICORICE FLAVONOIDS CONSTITUENTS PREVENTION PHARMACOLOGY ANTIOXIDANT Aldose Reductase Inhibitory Compounds from Glycyrrhiza uralensis Article 10.1248/bpb.33.917 1 BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.33, no.5, pp.917 - 921 BIOLOGICAL & PHARMACEUTICAL BULLETIN 33 5 917 921 scie scopus 000277012400032 2-s2.0-77952414610 Pharmacology & Pharmacy Pharmacology & Pharmacy Article ADVANCED GLYCATION ENDPRODUCTS DIABETIC COMPLICATIONS PHENOLIC-COMPOUNDS KK-A(Y) MICE LICORICE FLAVONOIDS CONSTITUENTS PREVENTION PHARMACOLOGY ANTIOXIDANT Glycyrrhiza uralensis semilicoisoflavone B aldose reductase diabetic complication polyol pathway