Lee, Seungduk Lee, Mina Koh, Dalkwon Kim, Beop-Min Choi, Jee Hyun 2024-01-20T19:31:37Z 2024-01-20T19:31:37Z 2021-09-02 2010-05 1083-3668 https://pubs.kist.re.kr/handle/201004/131519 We applied near-infrared spectroscopy (NIRS) and electroencephalography (EEG) simultaneously on the mouse brain and investigated the hemodynamic response to epileptic episodes under pharmacologically driven seizure. gamma-butyrolactone (GBL) and 4-aminopyridine (4-AP) were applied to induce absence and tonic-clonic seizures, respectively. The epileptic episodes were identified from the single-channel EEG, and the corresponding hemodynamic changes in different regions of the brain were characterized by multichannel frequency-domain NIRS. Our results are the following: (i) the oxyhemoglobin level increases in the case of GBL-treated mice but not 4-AP-treated mice compared to the predrug state; (ii) the dominant response to each absence seizure is a decrease in deoxyhemolobin; (iii) the phase shift between oxy- and deoxyhemoglobin reduces in GBL-treated mice but no 4-AP-treated mice; and (iv) the spatial correlation of hemodynamics increased significantly in 4-AP-treated mice but not in GBL-treated mice. Our results shows that spatiotemporal tracking of cerebral hemodynamics using NIRS can be successfully applied to the mouse brain in conjunction with electrophysiological recording, which will support the study of molecular, cellular, and network origin of neurovascular coupling in vivo. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI:10.1117/1.3365952] English SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS ABSENCE SEIZURES BLOOD-VOLUME EEG-FMRI HEMOGLOBIN EPILEPSY OXYGENATION TOPOGRAPHY PARAMETERS FREQUENCY CORTEX Cerebral hemodynamic responses to seizure in the mouse brain: simultaneous near-infrared spectroscopy-electroencephalography study Article 10.1117/1.3365952 1 JOURNAL OF BIOMEDICAL OPTICS, v.15, no.3 JOURNAL OF BIOMEDICAL OPTICS 15 3 scie scopus 000280642900052 2-s2.0-79958066876 Biochemical Research Methods Optics Radiology, Nuclear Medicine & Medical Imaging Biochemistry & Molecular Biology Optics Radiology, Nuclear Medicine & Medical Imaging Article ABSENCE SEIZURES BLOOD-VOLUME EEG-FMRI HEMOGLOBIN EPILEPSY OXYGENATION TOPOGRAPHY PARAMETERS FREQUENCY CORTEX mouse near-infrared spectroscopy electroencephalography seizure oxyhemoglobin deoxyhemoglobin