Kim, Eunju Hwang, Eun Mi Yarishkin, Oleg Yoo, Jae Cheal Kim, Donggyu Park, Nammi Cho, Minhee Lee, Young Sun Sun, Choong-Hyun Yi, Gwan-Su Yoo, Jiyun Kang, Dawon Han, Jaehee Hong, Seong-Geun Park, Jae-Yong 2024-01-20T19:31:53Z 2024-01-20T19:31:53Z 2022-01-10 2010-04-30 0006-291X https://pubs.kist.re.kr/handle/201004/131529 TREK1 belongs to a family of two-pore-domain K+ (K-2P) channels and produce background currents that regulate cell excitability. In the present study, we identified a vesicle transport protein, beta-COP, as an interacting partner by yeast two-hybrid screening of a human brain cDNA library with N-terminal region of TREK1 (TREK1-N) as bait. Several in vitro and in vivo binding assays confirmed the protein-protein interaction between TREK1 and beta-COP. We also found that beta-cop was associated with TREK1 in native condition at the PC3 cells. When RFP-beta-COP was co-transfected with GFP-TREK1 into COS-7 cells, both proteins were found localized to the plasma membrane. In addition, the channel activity and surface expression of GFP-TREK1 increased dramatically by co-transfection with RFP-beta-COP. Surface expression of the TREK1 channel was also clearly reduced with the addition of beta-COP-specific shRNA. Collectively, these data suggest that beta-COP plays a critical role in the forward transport of TREK1 channel to the plasma membrane. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE TRANSMEMBRANE CONDUCTANCE REGULATOR ENDOPLASMIC-RETICULUM POTASSIUM CHANNELS INDUCED INHIBITION ARACHIDONIC-ACID K+ CHANNELS TRAFFICKING TRANSPORT CARGO Enhancement of TREK1 channel surface expression by protein-protein interaction with beta-COP Article 10.1016/j.bbrc.2010.03.171 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.395, no.2, pp.244 - 250 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 395 2 244 250 scie scopus 000277681000015 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article TRANSMEMBRANE CONDUCTANCE REGULATOR ENDOPLASMIC-RETICULUM POTASSIUM CHANNELS INDUCED INHIBITION ARACHIDONIC-ACID K+ CHANNELS TRAFFICKING TRANSPORT CARGO TREK1 beta-COP Yeast two-hybrid screening Trafficking