Lee, Jung-Ok Kim, Chulyoung Lee, Seung-Woo Oak, Min-Ho 2024-01-20T19:31:56Z 2024-01-20T19:31:56Z 2021-09-02 2010-04-30 1976-9148 https://pubs.kist.re.kr/handle/201004/131531 Several studies have shown that plant-derived polyphenols reduce cardiovascular accidents in high-risk patients and the inhibition of platelet function may be responsible for part of this benefit. Lindera obtusiloba is widely used in traditional herbal medicine for the treatment of cardiovascular and inflammatory diseases. Therefore, the antiplatelet and antithrombotic activities of Lindera obtusiloba Extracts (LOE) on in vitro platelet aggregation, radical scavenging activity and in vivo murine pulmonary thrombosis were examined. LOE was able to directly scavenge the stable DPPH radical in a concentration-dependent manner and its IC50 value was 3.9 +/- 0.1 mu g/ml. LOE significantly inhibited collagen- and ADP-induced platelet aggregation in a concentration-dependent manner and its IC50 value is 0.9 +/- 0.1 mg/ml and 0.4 +/- 0.1 mg/ml respectively. The inhibitory effect of LOE was comparable to aspirin (IC50 values were 1.0 +/- 0.5 and 1.0 +/- 0.7 mg/ml, respectively). Furthermore, oral administration of LOE suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen plus epinephrine. Taken together, our results suggest LOE may be a promising candidate for antithrombotic agent, and the antithrombotic effect of LOE may be due to, at least in part, antiplatelet activity. English KOREAN SOC APPLIED PHARMACOLOGY INHIBIT PLATELET-FUNCTION NITRIC-OXIDE REDOX REGULATION THROMBOSIS AGGREGATION RECRUITMENT POLYPHENOLS MECHANISMS FLAVONOIDS THERAPY Antiplatelet and Antithrombotic Activities of Lindera obtusiloba Extract in vitro and in vivo Article 10.4062/biomolther.2010.18.2.205 1 BIOMOLECULES & THERAPEUTICS, v.18, no.2, pp.205 - 210 BIOMOLECULES & THERAPEUTICS 18 2 205 210 scie scopus kci 000277582000012 2-s2.0-77953098053 Biochemistry & Molecular Biology Pharmacology & Pharmacy Biochemistry & Molecular Biology Pharmacology & Pharmacy Article INHIBIT PLATELET-FUNCTION NITRIC-OXIDE REDOX REGULATION THROMBOSIS AGGREGATION RECRUITMENT POLYPHENOLS MECHANISMS FLAVONOIDS THERAPY L.obtusiloba Platelet Thrombosis Antioxidant