Jiang, Hu-Lin Kim, You-Kyoung Lee, Sun-Mi Park, Mi-Ran Kim, Eun-Mi Jin, Yong-Mei Arote, Rohidas Jeong, Hwan-Jeong Song, Soo-Chang Cho, Myung-Haing Cho, Chong-Su 2024-01-20T19:33:05Z 2024-01-20T19:33:05Z 2021-09-02 2010-04 0253-6269 https://pubs.kist.re.kr/handle/201004/131589 Hydrogels are widely used in drug delivery systems because they can control the release and thereby enhance the efficiency of locally delivered bioactive molecules such as therapeutic drugs, proteins, or genes. For gene delivery, localized release of plasmid DNA or polymer/DNA complexes can transfect cells and produce sustained protein production. We tested the galactosylated chitosan-graft-polyethylenimine (GC-g-PEI)/DNA complexes-loaded poly(organophosphazene) thermosensitive biodegradable hydrogel as a hepatocyte targeting gene delivery system. The poly(organophosphazene) hydrogel loaded with GC-g-PEI/DNA complexes showed low cytotoxicity and higher transfection efficiency than PEI/DNA complexes, as well as good hepatocyte specificity in vitro and in vivo. Our results indicate that poly(organophosphazene) hydrogels loaded with GC-g-PEI/DNA complexes may be a safe and efficient hepatocyte targeting gene delivery system. English PHARMACEUTICAL SOC KOREA DNA DELIVERY IN-VITRO BIOMEDICAL APPLICATIONS GRAFT-POLYETHYLENIMINE SCAFFOLDS CARRIER POLYPHOSPHAZENES MATRICES RELEASE Galactosylated Chitosan-g-PEI/DNA Complexes-loaded Poly(organophosphazene) Hydrogel as a Hepatocyte Targeting Gene Delivery System Article 10.1007/s12272-010-0409-9 1 ARCHIVES OF PHARMACAL RESEARCH, v.33, no.4, pp.551 - 556 ARCHIVES OF PHARMACAL RESEARCH 33 4 551 556 scie scopus kci ART001438345 000276970700010 2-s2.0-77954504897 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article DNA DELIVERY IN-VITRO BIOMEDICAL APPLICATIONS GRAFT-POLYETHYLENIMINE SCAFFOLDS CARRIER POLYPHOSPHAZENES MATRICES RELEASE Gene therapy Galactosylated chitosan-g-polyethylenimine Poly(organophosphazene) hydrogel Hepatocyte targeting Cytotoxicity Transfection efficiency