Lee, Kangsun Kim, Choong Kim, Youngeun Jung, Keunhui Ahn, Byungwook Kang, Ji Yoon Oh, Kwang W. 2024-01-20T19:33:15Z 2024-01-20T19:33:15Z 2021-09-02 2010-04 1387-2176 https://pubs.kist.re.kr/handle/201004/131597 We present a 2-layer based microfluidic concentration generator by a hybrid of a serial and a volumetric dilution for dose-response experiments in drug screening. The hybrid dilution method using 2-layer based microfluidic network significantly reduces the total number of cascaded serial dilution stages. The proposed strategy is capable of generating a large number of universal stepwise monotonic concentrations with a wide range of logarithmic and linear scales. We have studied an equivalent electrical circuit to that of the 2-layer based microfluidic network, where the only variable parameter is channel length. We have designed a microfluidic dilution generator simultaneously covering 14 doses with a combination of 4-order logarithmic and 4-point linear concentrations. The design has been verified by a commercial circuit analysis software (e.g., P-Spice) for the electrical circuit analysis and a computational fluid dynamics software (e.g., CFD-ACE(+)) for the microfluidic circuit analysis. As a real-life application of the proposed dilution generator, we have successfully performed a dose-response experiment using MCF-7 human breast cancer cells. We expect that the proposed dilution method will be useful to study not only high throughput drug screening but also optimization in biology, chemistry, medicine, and material sciences. English SPRINGER ON-A-CHIP CELL-CULTURE GRADIENTS PARALLEL SYSTEMS DEVICES ARRAYS 2-layer based microfluidic concentration generator by hybrid serial and volumetric dilutions Article 10.1007/s10544-009-9385-6 1 BIOMEDICAL MICRODEVICES, v.12, no.2, pp.297 - 309 BIOMEDICAL MICRODEVICES 12 2 297 309 scie scopus 000275459800013 2-s2.0-77952891144 Engineering, Biomedical Nanoscience & Nanotechnology Engineering Science & Technology - Other Topics Article ON-A-CHIP CELL-CULTURE GRADIENTS PARALLEL SYSTEMS DEVICES ARRAYS Serial dilution Volumetric dilution Drug screening Microfluidic network