Nam, Taehwan Park, Sangjin Lee, Seung-Young Park, Kyeongsoon Choi, Kuiwon Song, In Chan Han, Moon Hee Leary, James J. Yuk, Simseok Andrew Kwon, Ick Chan Kim, Kwangmeyung Jeong, Seo Young 2024-01-20T19:33:31Z 2024-01-20T19:33:31Z 2021-09-02 2010-04 1043-1802 https://pubs.kist.re.kr/handle/201004/131611 We report tumor targeting nanoparticles for optical/MR dual imaging based on self-assembled glycol chitosan to be a potential multimodal imaging probe. To develop an optical/MR dual imaging probe, biocompatible and water-soluble glycol chitosan (M-w = 50 kDa) were chemically modified with 5 beta-cholanic acid (CA), resulting in amphiphilic glycol chitosan-5 beta-cholanic acid conjugates (GC-CA). For optical imaging near-infrared fluorescence (NIRF) dye, Cy5.5, was conjugated to GC-CA resulting in Cy5-labeled GC-CA conjugates (Cy5.5-GC-CA). Moreover, in order to dictate gadolinium (Gd(III)) in the Cy5.5-GC-CA conjugates, 1,4,7,10-tetraazacyclododecane1,4,7,10-tetraacetic acid (DOTA) was directly conjugated in Cy5.5-GC-CA. Finally, the excess GdCl3 was added to DOTA modified Cy5.5-GC-CA conjugates in distilled water (pH 5.5). The freshly prepared Gd(III) encapsulated Cy5.5-GC-CA conjugates were spontaneously self-assembled into stable Cy5.5 labeled and Gd(III) encapsulated chitosan nanoparticles (Cy5.5-CNP-Gd(III)). The Cy5.5-CNP-Gd(III) was spherical in shape and approximately 350 nm in size. From the cellular experiment, it was demonstrated that Cy5.5-CNP-Gd(III) were efficiently taken up and distributed in cytoplasm (NIRF lilter; red). When the Cy5.5-GC-Gd(III) were systemically administrated into the tail vein of tumor-bearing mice, large amounts of nanoparticles were successfully localized within the tumor, which was confirmed by noninvasive near-infrared fluorescence and MR imaging system simultaneously. These results revealed that the dual-modal imaging probe of Cy5.5-CNP-Gd(III) has the potential to be used as an optical/MR dual imaging agent for cancer treatment. English AMER CHEMICAL SOC CONTRAST AGENTS QUANTUM DOTS IN-VIVO DRUG-DELIVERY THERAPY CARRIERS MR Tumor Targeting Chitosan Nanoparticles for Dual-Modality Optical/MR Cancer Imaging Article 10.1021/bc900408z 1 BIOCONJUGATE CHEMISTRY, v.21, no.4, pp.578 - 582 BIOCONJUGATE CHEMISTRY 21 4 578 582 scie scopus 000276817800002 2-s2.0-77951261362 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article CONTRAST AGENTS QUANTUM DOTS IN-VIVO DRUG-DELIVERY THERAPY CARRIERS MR