An, Yu Ri Kim, Seung-Jun Park, Hye-Won Oh, Moon-Ju Kim, Youn-Jung Ryu, Jae-Chun Hwang, Seung Yong 2024-01-20T19:33:52Z 2024-01-20T19:33:52Z 2021-09-02 2010-03-20 1976-0280 https://pubs.kist.re.kr/handle/201004/131625 Polycyclic aromatic hydrocarbons (PAHs) are one of the most widespread organic pollutants, and they can cause changes in gene expression. Biochip based microarray technologies are useful for identifying the toxicity of environmental pollutants. In this study, we performed a microarray experiment to identify the effects of PAHs in human hepatoma cells using the HazChem human array V3. The cells were exposed for 48 hours to IC20 values of four PAH chemicals (benzo[a]pyrene, dibenzo[a,h]anthracene, 3-methylcholanthrene, benzo[k]fluoranthene). Thirty of 1136 genes, which were related to the cell cycle based the ontology analysis, were commonly detected when cells were exposed to the four chemicals. Further analysis of the significantly expressed genes revealed that they affected the spindle check point. These results suggest that PAHs affect cell cycle regulation in humans. English KOREAN BIOCHIP SOCIETY-KBCS PROLIFERATION TOXICOLOGY Differential expression of cell cycle related genes in PAH-exposed human liver cells Article 10.1007/s13206-010-4105-0 1 BIOCHIP JOURNAL, v.4, no.1, pp.30 - 34 BIOCHIP JOURNAL 4 1 30 34 scie scopus kci other ART001428930 000275902400005 2-s2.0-79959752358 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article PROLIFERATION TOXICOLOGY PAHs HazChem Microarray Cell-cycle Hepatotoxicity