Kim, Yong-Hak Lee, Cheolju Go, Hayoung Konishi, Kyoko Lee, Kangseok Lau, Peter C. K. Yu, Myeong-Hee 2024-01-20T20:00:44Z 2024-01-20T20:00:44Z 2021-09-02 2010-02-15 0003-9861 https://pubs.kist.re.kr/handle/201004/131717 We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was determined by optical absorbance spectroscopy with an extinction coefficient (E(550-535)) of 19.7 +/- 6.3 mM(-1) cm(-1) and reduction potential of + 261 mV. When purified CeCyt was added into the mitochondria, the specific activity of CeCyt reached 440 +/- 122 mu mol min(-1) mg(-1) protein. The inhibition of MG reduction by stigmatellin, but not by antimycin A, indicated a possible linkage of CeCyt activity to the Qo site of the bc1 complex. The RT-PCR results showed tight regulation of the cecyt gene expression by reactive oxygen species. We suggest that CeCyt acts as a protein reductant for MG under oxidative stress in a stationary or secondary growth stage of this fungus. (C) 2009 Elsevier Inc. All rights reserved. English ELSEVIER SCIENCE INC LEUCOMALACHITE GREEN B6C3F(1) MICE TRIPHENYLMETHANE REDUCTASE GENTIAN-VIOLET TOXICITY RATS DYES CARCINOGENICITY DEHYDROGENASE RESPIRATION Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans Article 10.1016/j.abb.2009.11.027 1 ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, v.494, no.2, pp.159 - 165 ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 494 2 159 165 scie scopus 000274745900007 2-s2.0-75549087544 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article LEUCOMALACHITE GREEN B6C3F(1) MICE TRIPHENYLMETHANE REDUCTASE GENTIAN-VIOLET TOXICITY RATS DYES CARCINOGENICITY DEHYDROGENASE RESPIRATION Malachite green Leucomalachite green Triphenylmethane dyes Cytochrome c Cunninghamella elegans