Shalek, Alex K. Robinson, Jacob T. Karp, Ethan S. Lee, Jin Seok Ahn, Dae-Ro Yoon, Myung-Han Sutton, Amy Jorgolli, Marsela Gertner, Rona S. Gujral, Taranjit S. MacBeath, Gavin Yang, Eun Gyeong Park, Hongkun 2024-01-20T20:00:50Z 2024-01-20T20:00:50Z 2021-09-02 2010-02-02 0027-8424 https://pubs.kist.re.kr/handle/201004/131723 A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, and universal delivery of biomolecules into immortalized and primary mammalian cells using surface-modified vertical silicon nanowires. The method relies on the ability of the silicon nanowires to penetrate a cell's membrane and subsequently release surface-bound molecules directly into the cell's cytosol, thus allowing highly efficient delivery of biomolecules without chemical modification or viral packaging. This modality enables one to assess the phenotypic consequences of introducing a broad range of biological effectors (DNAs, RNAs, peptides, proteins, and small molecules) into almost any cell type. We show that this platform can be used to guide neuronal progenitor growth with small molecules, knock down transcript levels by delivering siRNAs, inhibit apoptosis using peptides, and introduce targeted proteins to specific organelles. We further demonstrate codelivery of siRNAs and proteins on a single substrate in a microarray format, highlighting this technology's potential as a robust, monolithic platform for high-throughput, miniaturized bioassays. English NATL ACAD SCIENCES STEM-CELLS HIPPOCAMPAL-NEURONS CARBON NANOTUBES SIRNA DELIVERY MICROARRAYS ARRAYS LITHOGRAPHY FABRICATION INTEGRATION ASTROCYTES Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells Article 10.1073/pnas.0909350107 1 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.107, no.5, pp.1870 - 1875 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107 5 1870 1875 scie scopus 000274296300015 2-s2.0-76649092675 Multidisciplinary Sciences Science & Technology - Other Topics Article STEM-CELLS HIPPOCAMPAL-NEURONS CARBON NANOTUBES SIRNA DELIVERY MICROARRAYS ARRAYS LITHOGRAPHY FABRICATION INTEGRATION ASTROCYTES intracellular delivery microarray high-throughput bioassay nanobiotechnology