Joo, Jong Wha J. Na, Seungjin Baek, Je-Hyun Lee, Cheolju Paek, Eunok 2024-01-20T20:01:06Z 2024-01-20T20:01:06Z 2022-01-10 2010-02 1535-3893 https://pubs.kist.re.kr/handle/201004/131735 Shotgun proteomics using mass spectrometry (MS) has become the choice for large-scale peptide and protein identification. The recent development of high-resolution mass spectrometers such as FT-ICR or Orbitrap makes it possible to identify peptides within only a few parts per million (ppm), and it is expected to dramatically improve performance of peptide identification, as compared to low-resolution instruments. To fully exploit such significantly higher mass accuracy, however, appropriate data analysis methods are required. Here, we present a new target-decoy strategy, called Target-Decoy with Mass Binning, utilizing high mass accuracy for peptide identification validation, which remains a challenging problem in MS-based proteomics. When tested on various high-resolution MS data, our method was very effective and yet simple and showed comparable or better performance when compared with other validation methods. English AMER CHEMICAL SOC PEPTIDE IDENTIFICATION SEQUENCE DATABASES SEARCH STRATEGY ACCURACY MODEL Target-Decoy with Mass Binning: A Simple and Effective Validation Method for Shotgun Proteomics Using High Resolution Mass Spectrometry Article 10.1021/pr9006377 1 JOURNAL OF PROTEOME RESEARCH, v.9, no.2, pp.1150 - 1156 JOURNAL OF PROTEOME RESEARCH 9 2 1150 1156 scie scopus 000274269400051 2-s2.0-76149104250 Biochemical Research Methods Biochemistry & Molecular Biology Article PEPTIDE IDENTIFICATION SEQUENCE DATABASES SEARCH STRATEGY ACCURACY MODEL target-decoy peptide identification validation method precursor mass error mass binning high-resolution mass spectrometry