Cha, Kwang Hyun Lee, Hee Ju Koo, Song Yi Song, Dae-Geun Lee, Dong-Un Pan, Cheol-Ho 2024-01-20T20:01:53Z 2024-01-20T20:01:53Z 2021-09-02 2010-01-27 0021-8561 https://pubs.kist.re.kr/handle/201004/131771 Pressurized liquid extraction (PLE) was applied to the extraction of carotenoids and chlorophylls from the green microalga Chlorella vulgaris. Four extraction techniques such as maceration (MAC), Soxhlet extraction (SOX), ultrasound assisted extraction (UAE), and PLE were compared, and both the extraction temperature (50, 105, and 160 degrees C) and the extraction time (8, 19, and 30 min), which are the two main factors for PILE, were optimized with a central composite design to obtain the highest extraction efficiency. The extraction solvent (90% ethanol/water) could adequately extract the functional components from C. vulgaris. PLE showed higher extraction efficiencies than MAC, SOX, and UAE. Temperature was the key parameter having the strongest influence on the extraction of carotenoids and chlorophylls from chlorella. In addition, high heat treatment (>110 degrees C) by PLE minimized the formation of pheophorbide a, a harmful chlorophyll derivative. These results indicate that PLE may be a useful extraction method for the simultaneous extraction of carotenoids and chlorophylls from C. vulgaris. English AMER CHEMICAL SOC ACCELERATED SOLVENT-EXTRACTION SUPERCRITICAL CO2 EXTRACTION DUNALIELLA-SALINA FLUID EXTRACTION PHEOPHYTIN MICROALGAE PHEOPHORBIDE ANTIOXIDANTS DERIVATIVES ALGA Optimization of Pressurized Liquid Extraction of Carotenoids and Chlorophylls from Chlorella vulgaris Article 10.1021/jf902628j 1 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.58, no.2, pp.793 - 797 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 58 2 793 797 scie scopus 000273671900017 2-s2.0-74849138750 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article ACCELERATED SOLVENT-EXTRACTION SUPERCRITICAL CO2 EXTRACTION DUNALIELLA-SALINA FLUID EXTRACTION PHEOPHYTIN MICROALGAE PHEOPHORBIDE ANTIOXIDANTS DERIVATIVES ALGA Pressurized liquid extraction carotenoids chlorophylls Chlorella vulgaris optimization