Kim, Dong-Gyu Hwang, Eun Mi Yoo, Jae Cheal Kim, Eunju Park, Nammi Rhee, Sangmyung Ha, Chang Man Hong, Seong-Geun Park, Jae-Yong 2024-01-20T20:02:21Z 2024-01-20T20:02:21Z 2021-09-01 2010-01-01 0006-291X https://pubs.kist.re.kr/handle/201004/131794 NELL2 is a neuron-specific secreted glycoprotein containing in N-terminal thrombospondin I-like domain (TSP-N) In this study. we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2 NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain NELL2-Tsp is a glycosylated protein specifically expressed In brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However. Clue to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2 Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite Outgrowth These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2 (C) 2009 Elsevier Inc All rights reserved English ACADEMIC PRESS INC ELSEVIER SCIENCE PROTEIN GROWTH DOMAIN EXPRESSION NEURONS Identification and characterization of a truncated isoform of NELL2 Article 10.1016/j.bbrc.2009.11.092 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.391, no.1, pp.529 - 534 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 391 1 529 534 scie scopus 000273624500093 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article PROTEIN GROWTH DOMAIN EXPRESSION NEURONS NELL2 Truncated NELL2 Alternative splicing Neurite outgrowth Secretion