Yum, Seungshic Woo, Seonock Kagami, Yoshihiro Park, Hong-Seog Ryu, Jae-Chun 2024-01-20T20:03:25Z 2024-01-20T20:03:25Z 2021-09-02 2010-01 1532-0456 https://pubs.kist.re.kr/handle/201004/131849 Differential gene expression profiling was performed with a cDNA microarray in the liver tissue of the medaka fish, Oryzias latipes, after exposure to Arochlor 1260, a polychlorinated biphenyl (PCB) mixture, which is used as a coolant and insulating fluid for transformers and capacitors and is classified as a persistent organic pollutant. Twenty-six differentially expressed candidate genes were identified. The expression of 12 genes was up-regulated and that of 14 genes was down-regulated. These genes are associated with the cytoskeleton, development, endocrine/reproduction, immunity, metabolism, nucleic acid/protein binding, and signal transduction, or are uncategorized. The transcription of molecular biomarkers known to be involved in endocrine disruption (e.g., vitellogenins, choriogenins, and estrogen receptor alpha) was highly upregulated. The same tendencies in gene expression changes were observed with real-time quantitative PCR (qRT-PCR) analysis, which was conducted to examine 12 selected candidate genes. These genes could be used as molecular biomarkers for biological responses to toxic chemicals, especially endocrine disrupting and carcinogenic chemical contamination in aquatic environments. (C) 2009 Elsevier Inc. All rights reserved. English ELSEVIER SCIENCE INC ARYL-HYDROCARBON RECEPTOR SQUAMOUS-CELL CARCINOMA EGG ENVELOPE CHORION ORYZIAS-LATIPES ESTROGEN-RECEPTOR JAPANESE MEDAKA MOLECULAR-CLONING COMPLEMENT COMPONENTS MICROARRAY ANALYSIS KERATIN EXPRESSION Changes in gene expression profile of medaka with acute toxicity of Arochlor 1260, a polychlorinated biphenyl mixture Article 10.1016/j.cbpc.2009.08.007 1 COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v.151, no.1, pp.51 - 56 COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 151 1 51 56 scie scopus 000273152400006 2-s2.0-71649112072 Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology Article ARYL-HYDROCARBON RECEPTOR SQUAMOUS-CELL CARCINOMA EGG ENVELOPE CHORION ORYZIAS-LATIPES ESTROGEN-RECEPTOR JAPANESE MEDAKA MOLECULAR-CLONING COMPLEMENT COMPONENTS MICROARRAY ANALYSIS KERATIN EXPRESSION Oryzias latipes Arochlor 1260 Polychlorinated biphenyls (PCBs) Differential gene expression profile Real-time quantitative PCR cDNA microarray