Hwang, Jae-Hoon Choi, Jeong-A Abou-Shanab, R. A. I. Bhatnagar, Amit Min, Booki Song, Hocheol Kumar, Eva Choi, Jaeyoung Lee, Eung Seok Kim, Yong Je Um, Sukkee Lee, Dae Sung Jeon, Byong-Hun 2024-01-20T20:04:46Z 2024-01-20T20:04:46Z 2021-09-05 2009-12 0360-3199 https://pubs.kist.re.kr/handle/201004/131912 The effects of varying sulfate concentrations with pH on continuous fermentative hydrogen production were studied using anaerobic mixed cultures growing on a glucose substrate in a chemostat reactor. The maximum hydrogen production rate was 2.8 L/day at pH 5.5 and sulfate concentration of 3000 mg/L. Hydrogen production and residual sulfate level decreased with increasing the pH from 5.5 to 6.2. The volatile fatty acids (VFAs) and ethanol fractions in the effluent were in the order of butyric acid (HBu) > acetic acid (HAc) > ethanol > propionic acid (HPr). Fluorescence In Situ Hybridization (FISH) analysis revealed the presence of hydrogen producing bacteria (HPB) under all pH ranges while sulfate reducing bacteria (SRB) were present at pH 5.8 and 6.2. The inhibition in hydrogen production by SRB at pH 6.2 diminished entirely by lowering to pH 5.5, at which activity of SRB; is substantially suppressed. (C) 2009 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD METHANE-PRODUCING BACTERIA PRODUCTION WASTE-WATER REDUCING BACTERIA FULL-SCALE REDUCTION ACIDOGENESIS TEMPERATURE INHIBITION SUBSTRATE DIGESTION Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora Article 10.1016/j.ijhydene.2009.10.022 1 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v.34, no.24, pp.9702 - 9710 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 34 24 9702 9710 scie scopus 000272856400008 2-s2.0-71749119182 Chemistry, Physical Electrochemistry Energy & Fuels Chemistry Electrochemistry Energy & Fuels Article METHANE-PRODUCING BACTERIA PRODUCTION WASTE-WATER REDUCING BACTERIA FULL-SCALE REDUCTION ACIDOGENESIS TEMPERATURE INHIBITION SUBSTRATE DIGESTION Hydrogen production Anaerobic mixed microflora pH Sulfate Fluorescence in situ hybridization