Park, In-Su Han, Min Rhie, Jong-Won Kim, Soo Hyun Jung, Youngmee Kim, Ik Hwan Kim, Sang-Heon 2024-01-20T20:05:13Z 2024-01-20T20:05:13Z 2021-09-04 2009-12 0142-9612 https://pubs.kist.re.kr/handle/201004/131934 In recent years, research in the areas of stem cells has dramatically increased, including studies of cellular adhesion to a substrate. We sought to determine the adhesive properties of human adipose-derived stem cells (hASCs) for extracellular matrix proteins. The adhesion of hASCs to collagens and laminin was completely inhibited by a monoclonal antibody, Mab 2253, which binds to the beta 1 integrin subunit. These data indicate that hASC adhesion to collagens and laminin was exclusively mediated by an integrin. Cell adhesion on fibronectin (Fn) was inhibited by the heparin-binding peptide (HBP) in the presence of Mab 2253, but not by either Mab 2253 or HBP alone. These results indicate that both the beta 1 subunit and the heparan sulfate proteoglycam participated in the cell adhesion to Fn. Microscopic views showed extensive spreading of hASCs cultured on Fn, whereas the cells maintained a round shape when cultured on a heparin-binding domain (HBD) substrate. hASCs differentiated into adipocytes, which stained positive for lipid vacuoles by Oil Red-O analysis, more readily on HBD substrate than on FN substrate. These results suggest that hASCs have an adhesion mechanism for the HBD of Fn and hASC morphology is controlled by the adhesion mechanism and strongly correlated with adipogenic differentiation. (C) 2009 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD HEPARIN-BINDING DOMAIN MATRIX-MEDIATED RETENTION BONE-MARROW OSTEOGENIC DIFFERENTIATION MALTOSE-BINDING FIBRONECTIN SURFACE RECEPTOR PROTEIN PROLIFERATION The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism Article 10.1016/j.biomaterials.2009.08.057 1 BIOMATERIALS, v.30, no.36, pp.6835 - 6843 BIOMATERIALS 30 36 6835 6843 scie scopus 000272071400004 2-s2.0-70350346591 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article HEPARIN-BINDING DOMAIN MATRIX-MEDIATED RETENTION BONE-MARROW OSTEOGENIC DIFFERENTIATION MALTOSE-BINDING FIBRONECTIN SURFACE RECEPTOR PROTEIN PROLIFERATION Cell adhesion substrate Human adipose-derived stem cells Extracellular matrix Fibronectin Heparin-binding domain Cell differentiation