Kim, Chul Young Lee, Hee Ju Jung, Sang Hoon Lee, Eun Ha Cha, Kwang Hyun Kang, Suk Woo Pan, Cheol-Ho Um, Byung-Hun 2024-01-20T20:05:14Z 2024-01-20T20:05:14Z 2021-09-04 2009-12 1738-2203 https://pubs.kist.re.kr/handle/201004/131935 The methanol extract of black bamboo (Phyllostachys nigra Munro) leaves showed significant radical scavenging activity in an ABTS(+)-based assay. The high-performance liquid chromatography (HPLC) coupled to an online ABTS(+)-based assay system was used to identify and quantify the antioxidant principles from bamboo leaves. Antioxidant compounds were isolated from the ethyl acetate soluble fraction through the activity profiles based on the online A-BTS+ assay, and identified as isoorientin (1), orientin (2), vitexin (3), luteolin 6-C-(6-O-trans-caffeoylglucoside) (4), vittariflavone (5), and tricin (6) using NMR and H-PLGESUMS data. Antioxidant activity levels of the isolated isoorientin (1), orientin (2), and luteolin 6-C-(6-O-trans-caffeoylglucoside) (4) were 33.10, 30.96, and 17.11 mM for a 50% reduction of the ABTS(+) radical, respectively. This HPLC online ABTS'-based assay system was a rapid and efficient method for measuring antioxidants' activity-guided purification from natural resources. English KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY ANTIOXIDANT ACTIVITY FLAVONOIDS TOXICOLOGY TOXICITY SAFETY HPLC Rapid Identification of Radical Scavenging Phenolic Compounds from Black Bamboo Leaves using High-Performance Liquid Chromatography Coupled to an Online ABTS(+)-Based Assay Article 10.3839/jksabc.2009.102 1 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.52, no.6, pp.613 - 619 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY 52 6 613 619 scie kci ART001411904 000274066900006 Food Science & Technology Food Science & Technology Article ANTIOXIDANT ACTIVITY FLAVONOIDS TOXICOLOGY TOXICITY SAFETY HPLC antioxidant flavonoids online antioxidants screening system Phyllostachys nigra