Baek, Ji Hye Gu, Man Bock Sang, Byoung-In Kwack, Seung Jun Kim, Kyu Bong Lee, Byung Mu 2024-01-20T20:05:25Z 2024-01-20T20:05:25Z 2021-09-03 2009-12 1528-7394 https://pubs.kist.re.kr/handle/201004/131943 Di-(2-ethylhexyl) phthalate (DEHP), one of the major phthalates, was reported to be a suspected endocrine-disrupting chemical (EDC) that might produce developmental or reproductive toxicities. Therefore, much effort was undertaken to reduce the potential risk of adverse effects of DEHP on humans by diminishing environmental exposure to this chemical. A bacterium was isolated from soil contaminated with DEHP at a poly(vinyl chloride) (PVC) manufacturing site. Biodegradation kinetic experiments on DEHP-contaminated soil samples were performed in a slurry phase system. The DEHP concentration was decreased to a concentration of 0.5 g/kg by the addition of 1% culture medium to the soil. The microorganism degraded DEHP through the formation of a mono-2-ethylhexyl phthalate (MEHP), which was subsequently metabolized to phthalic acid (PA), as detected by GC-MS analysis. Micrococcus luteus was able to degrade almost 90% of the initial DEHP within 12 d. In addition, the microbial toxicity study of DEHP and its degradation products MEHP or PA, using recombinant bioluminescent bacteria, showed that PA or the mixture produced protein or DNA damage. Data thus suggest that a new strain of Micrococcus luteus with a strong ability to degrade DEHP into nontoxic metabolites may contribute to decontamination of environmental phthalates and consequently risk reduction of human exposure to DEHP. English TAYLOR & FRANCIS INC BUTYL BENZYL PHTHALATE ENDOCRINE-DISRUPTING CHEMICALS ESCHERICHIA-COLI STRAIN DI(2-ETHYLHEXYL)PHTHALATE DEHP DI-2-ETHYLHEXYL PHTHALATE MANGROVE SEDIMENT OXIDATIVE STRESS ACID-ESTERS IN-VITRO PLASTICIZERS Risk Reduction of Adverse Effects Due to Di-(2-Ethylhexyl) Phthalate (DEHP) by Utilizing Microbial Degradation Article 10.1080/15287390903212733 1 JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v.72, no.21-22, pp.1388 - 1394 JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES 72 21-22 1388 1394 scie scopus 000271435300018 2-s2.0-75749090843 Environmental Sciences Public, Environmental & Occupational Health Toxicology Environmental Sciences & Ecology Public, Environmental & Occupational Health Toxicology Article BUTYL BENZYL PHTHALATE ENDOCRINE-DISRUPTING CHEMICALS ESCHERICHIA-COLI STRAIN DI(2-ETHYLHEXYL)PHTHALATE DEHP DI-2-ETHYLHEXYL PHTHALATE MANGROVE SEDIMENT OXIDATIVE STRESS ACID-ESTERS IN-VITRO PLASTICIZERS