Han, Min Park, In Su Kim, Soo Hyun Kim, Byung Soo Kim, Sang-Heon 2024-01-20T20:31:11Z 2024-01-20T20:31:11Z 2021-09-05 2009-11 0141-5492 https://pubs.kist.re.kr/handle/201004/131989 The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm(2) at 100 mu g protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces. English SPRINGER ESCHERICHIA-COLI SURFACE IMMOBILIZATION SOLUBILITY Design and characterization of a maltose binding protein-linked growth factor for matrix engineering Article 10.1007/s10529-009-0060-z 1 BIOTECHNOLOGY LETTERS, v.31, no.11, pp.1677 - 1684 BIOTECHNOLOGY LETTERS 31 11 1677 1684 scie scopus 000270646800005 2-s2.0-70350271180 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article ESCHERICHIA-COLI SURFACE IMMOBILIZATION SOLUBILITY Artificial matrix Cell adhesion Growth factor Maltose-binding protein