Chun, ChangJu Lim, Hye Jin Hong, Ki-Yun Park, Keun-Hong Song, Soo-Chang 2024-01-20T20:31:15Z 2024-01-20T20:31:15Z 2021-09-05 2009-11 0142-9612 https://pubs.kist.re.kr/handle/201004/131993 An injectable and thermosensitive poly(organophosphazene)-RGD conjugate to enhance functionality was synthesized by a covalent amide linkage between a cell adhesion peptide, GRGDS and carboxylic acid-terminated poly(organophosphazene). The aqueous solutions of synthesized poly(organophosphazene)GRGDS conjugates existed in an injectable fluid state at room temperature and immediately formed a hydrogel at body temperature. The rabbit mesenchymal stem cells (rMSCs) on the polymer-GRGDS conjugate (conjugate 1-2,0.05 mol fraction as GRGDS) hydrogel constructs using an injection method into a nude mouse were proved to express markers at mRNA level for all stages towards osteogenesis and mainly a sharp increase of osteocalcin (OCN, a typical late osteogenic differentiation marker) levels at 4th week post-induction indicated that the maturation process has started within this period. By histological and immunohistochemical evaluations, significantly high mineralization level by calcium contents was detected qualitatively and collagen type I (Col I), a major characteristic marker protein, was mainly and highly expressed by the rMSCs cultivated in the polymer-GRGDS conjugate hydrogel constructs formed into the nude mouse. The results suggest that the poly(organophosphazene)-GRGDS conjugate to enhance biofunctionality hold a promise for cell delivery material to induce osteogenic differentiation of MSC for enhancing ectopic bone formation. (C) 2009 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD TISSUE-ENGINEERING APPLICATIONS DRUG-DELIVERY EXTRACELLULAR-MATRIX CONTROLLED-RELEASE ADHESION PEPTIDES HYDROGELS BONE RGD POLYPHOSPHAZENES COPOLYMERS The use of injectable, thermosensitive poly(organophosphazene)-RGD conjugates for the enhancement of mesenchymal stem cell osteogenic differentiation Article 10.1016/j.biomaterials.2009.08.011 1 BIOMATERIALS, v.30, no.31, pp.6295 - 6308 BIOMATERIALS 30 31 6295 6308 scie scopus 000270767300016 2-s2.0-69649086765 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article TISSUE-ENGINEERING APPLICATIONS DRUG-DELIVERY EXTRACELLULAR-MATRIX CONTROLLED-RELEASE ADHESION PEPTIDES HYDROGELS BONE RGD POLYPHOSPHAZENES COPOLYMERS Thermosensitive Injectable Polyphosphazene-RGD conjugate Mesenchymal stem cells (MSCs) Osteogenic differentiation Hydrogel