Hye-Yeon Im Hye-Sil Park Hyun-Ah Choo Ae Nim Pae Oh-Seung Kwon 2024-01-20T20:32:55Z 2024-01-20T20:32:55Z 2021-09-06 2009-10 2093-5552 https://pubs.kist.re.kr/handle/201004/132071 5-HT2C receptors have been considered as therapeutic targets for the treatment of various central nervous system disorders such as depression, anxiety, epilepsy, schizophrenia, and sleep disorders. We chemically synthesized KKHQ80114 (K14) and KKHQ80109 (K09), selective 5-HT2C agonists, with the purpose of developing therapeutic agents for the treatment of obesity. The objective of this work is to investigate analytical methods of these compounds in the plasma and urine of rats by gas chromatography/mass spectrometry. In this experiment, K14 was determined in plasma and urine by using K09 as internal standard. Calibration curves give a good linearity in plasma (r2=0.9993) and urine (r2=0.9988). Among hexane, ethyl acetate and diethyl ether, the highest peak was observed in diethyl ether. However, ethyl acetate was used since more interfering peaks were observed with diethyl ether. Inter-day precision and accuracy were determined in the ranges of 50-500 ng/ml for plasma and 10-500 ng/ml for urine. Quantitation limits were 50 ng/ml plasma and 25 ng/ml urine. These data may be applicable for further studies of these compounds including absorption and metabolism due to no pharmacokinetic or analytical data available. English 한국약제학회 Determination of 5-HT2C agonist KKHQ80114 and KKHQ80109 in Rat Plasma and Urine by Gas-Chromatography/Mass Spectrometry Article 2 Journal of Pharmaceutical Investigation, v.39, no.5, pp.321 - 325 Journal of Pharmaceutical Investigation 39 5 321 325 N kci ART001385061 KKHQ80114 KKHQ80109 Gas chromatography/mass selective detector Plasma Urine Rats.