Jeon, Eugene Hyeon, Jeong-eun Suh, Dong Jin Suh, Young-Woong Kim, Seoung Wook Song, Kwang Ho Han, Sung Ok 2024-01-20T20:33:04Z 2024-01-20T20:33:04Z 2021-09-05 2009-10 1016-8478 https://pubs.kist.re.kr/handle/201004/132078 Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and beta-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an alpha-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and beta-glucosidase was able to produce ethanol from beta-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst. English KOREAN SOC MOLECULAR & CELLULAR BIOLOGY RECOMBINANT KLEBSIELLA-OXYTOCA AMORPHOUS CELLULOSE MICROCRYSTALLINE CELLULOSE CRYSTALLINE CELLULOSE YEAST-STRAIN FERMENTATION DEGRADATION HYDROLYSIS CELLULASES CELLOBIOSE Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera beta-glucosidase genes Article 10.1007/s10059-009-0131-y 1 MOLECULES AND CELLS, v.28, no.4, pp.369 - 373 MOLECULES AND CELLS 28 4 369 373 scie scopus kci ART001385727 000271987300007 2-s2.0-72449144777 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article RECOMBINANT KLEBSIELLA-OXYTOCA AMORPHOUS CELLULOSE MICROCRYSTALLINE CELLULOSE CRYSTALLINE CELLULOSE YEAST-STRAIN FERMENTATION DEGRADATION HYDROLYSIS CELLULASES CELLOBIOSE beta-glucosidase cellulose degradation Clostridium thermocellum endoglucanase ethanol production extracellular expression Saccharomyces cerevisiae