Lee, Eun Ha Song, Dae-Geun Lee, Joo Young Pan, Cheol-Ho Um, Byung Hun Jung, Sang Hoon 2024-01-20T20:33:11Z 2024-01-20T20:33:11Z 2021-09-05 2009-10 1738-2203 https://pubs.kist.re.kr/handle/201004/132084 The purpose of this study is to evaluate the active properties of Thuja orientalis leaves for the treatment of diabetic complications. The ethyl acetate fraction showed a significantly higher inhibition both of recombinant human aldose reductase (rhALR2) inhibitory activity and advanced glycation endproducts (AGEs). The detected antioxidants (compounds 14) by online-HPLC-ABTS(+) method and other three compounds were isolated using preparative RP-HPLC and Sephadex LM-20 column chromatography. Among the seven compounds, compound 4 (quercitrin) which was isolated from ethyl acetate fraction, was found to show inhibition for both forms of rhALR2 and AGEs. This compound also inhibited oxidative stress which was measured by Photochem (R) apparatus. In conclusion, the ethyl acetate fraction from T orientalis demonstrated antioxidant activity as well as inhibitory effects on rhALR2 and AGEs. Quercitrin was shown to be the active compound and hence could be offered as an active material of standardization for the development of natural products for food or medicines. English KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY DIABETIC COMPLICATIONS STRUCTURAL REQUIREMENTS LIQUID-CHROMATOGRAPHY ANTIOXIDANT ACTIVITY PROTEIN GLYCATION ONLINE SORBINIL Flavonoids from the Leaves of Thuja orientalis Inhibit the Aldose Reductase and the Formation of Advanced Glycation Endproducts Article 10.3839/jksabc.2009.078 1 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.52, no.5, pp.448 - 455 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY 52 5 448 455 scie kci ART001389266 000272062600007 Food Science & Technology Food Science & Technology Article DIABETIC COMPLICATIONS STRUCTURAL REQUIREMENTS LIQUID-CHROMATOGRAPHY ANTIOXIDANT ACTIVITY PROTEIN GLYCATION ONLINE SORBINIL advanced glycation endproducts aldose reductase online-HPLC-ABTS(+) method quercitrin Thuja orientalis