Jeon, Young Eun Lee, Yeon Sil Lim, Soon Sung Kim, Sun-Ju Jung, Sang Hoon Bae, Young-Soo Yi, Jae-Seon Kang, Il-Jun 2024-01-20T20:33:31Z 2024-01-20T20:33:31Z 2021-09-05 2009-10 1738-2203 https://pubs.kist.re.kr/handle/201004/132101 Phellinus linteus has traditionally been used as a medicinal mushroom in Oriental countries. Bioactive phytochemicals from the fruiting body of P. linteus were investigated using the on-line HPLC-DPPH system. The system was used to separate 10 antioxidants: protocatechuic acid (1) protocatechualdehyde, (2) caffeic acid (3), ellagic acid (4), hispidin (5), davalliallactone (6), interfungins A (7), hypholomine B (8), inoscavin A (9), and methyldavallialactone (10). Of these, compounds 6 and 7 showed the strongest inhibitory effect against the DPPH radical and superoxide anion radical-scavenging capacity. On comparison with quercetin (IC50 44.0 mu M), the IC50 value of compounds 6 and 7 for DPPH radical-scavenging capacity were 19.6 mu M and 18.5 mu M. Moreover, the antioxidative capacity of compound 6 at 40 mM was 2.95 nmol Trolox equivalents per mg dry wt. These results indicated that hispidin dimers such as hispidin with hispidin or hispolon had a stronger effect on radical-scavenging capacity than hispidin itself. English KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY RADICAL SCAVENGING COMPOUNDS ALDOSE REDUCTASE HISPIDIN L. CONSTITUENTS INHIBITOR CELLS Evaluation of the Antioxidant Activity of the Fruiting Body of Phellinus linteus Using the On-line HPLC-DPPH Method Article 10.3839/jksabc.2009.081 1 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.52, no.5, pp.472 - 479 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY 52 5 472 479 scie kci ART001389274 000272062600010 Food Science & Technology Food Science & Technology Article RADICAL SCAVENGING COMPOUNDS ALDOSE REDUCTASE HISPIDIN L. CONSTITUENTS INHIBITOR CELLS anti-oxidants DPPH radicals on-line RP-HPLC-DPPH Phellinus linteus superoxide anion radical-scavenging capacity